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胚胎脱胎率在植入前遗传测试对形积分 (PGT-A):从DoLoRes研究的回顾性数据分析

Barbara Wirleitner¹, Martina Hrubá^2,3, Maximilian Schuff⁴

¹Next Fertility IVF Prof. Zech, Roemerstrasse 2, 6900, Bregenz, Austria. Barbara.wirleitner@ivf.at.

Journal of assisted reproduction and genetics

|October 25, 2023

概括

由于各种因素,对阳积体 (PGT-A) 的植入前遗传检测显著减少可移植胚胎,特别是在晚年孕产妇患者中. 患者面临的高风险是没有euploid胚胎进行移植,需要对结果和成本的知情同意.

科学领域:

生殖医学生殖医学
人类遗传学人类遗传学
胚胎学胚胎学

背景情况:

前植入基因测试 (PGT-A) 旨在通过选择euploid胚胎来改善试管婴儿的结果.
然而,在PGT-A周期期间的胚胎损失可以显著影响治疗的成功.

研究的目的:

量化PGT-A周期中的胚胎损失,这些损失归因于非生物危险性质量,遗传分析失败和染色体异常.
评估这些损失对可移植胚胎可用性的影响.

主要方法:

从363个受控的卵巢刺激周期中对1562个芽细胞进行了回顾性多中心研究.
纳入标准:PGT-A循环,包括多叶体皮活检 (TB) 和下一代测序 (NGS).

主要成果:

由于胚胎质量差,损失了25.8%的胚胎囊.
10.3%的经过活检的胚胎囊遗传分析失败了.
只有18.1%的产生的胚胎囊是胚胎;50.4%的患者和57.6%的周期导致没有可转移的胚胎.
胚胎损失风险随着卵细胞产量降低和母亲年龄增加而增加.

结论:

PGT-A与实质性的胚胎脱落率有关,导致没有可转移的euploid胚胎的高风险.
晚期孕产妇年龄的患者尤其受到影响.
关于不确定的/马赛克结果,胚胎丢失和成本的知情同意对于接受PGT-A的患者至关重要.

关键词:

形积分症 (Aneuploidy) 是一种无形积分症.辅助生殖技术 (ART) 是一种辅助生殖技术.染色体的马赛克主义退学率的下降率是什么在植入前对阳积体的基因检测 (PGT-A)细分错误是指细分的错误.托菲克多德的活检 (TB)

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