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相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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相关实验视频

Updated: Jul 12, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
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Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

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在人类冠状动脉神经血管膜内的基因表达使用空间转录学.

Andrew P Voigt1,2, Nathaniel K Mullin1,2, Emma M Navratil1,2

  • 1Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, Iowa, United States.

Investigative ophthalmology & visual science
|October 25, 2023
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概括
此摘要是机器生成的。

这项研究使用空间RNA测序来揭示斑点新血管化中的改变基因表达,识别了参与这种视力威胁状况的关键分子和细胞类型.

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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相关实验视频

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Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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科学领域:

  • 眼科医生 眼科 眼科
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 黄斑新血管化 (MNV) 是与年龄相关的黄斑变性视力丧失的主要原因.
  • 在MNV中,病理性血管生成可以从胆膜或视网膜产生,对细胞特异性失调的理解有限.
  • 了解细胞机制对于开发有效的MNV治疗至关重要.

研究的目的:

  • 为了空间分析人类黄斑新血管化的基因表达变化.
  • 在MNV中识别失调的基因及其细胞起源.
  • 为了研究视网膜和胸膜中的区域基因表达模式.

主要方法:

  • 空间RNA测序是在使用MNV和健康对照对人类捐赠眼睛进行的.
  • 不同基因表达分析在MNV区域中发现了丰富的基因.
  • 解卷算法预测了负责基因表达变化的细胞类型.

主要成果:

  • 在MNV中的内皮细胞显示Rho家族GTPase和整体素信号基因的表达增加.
  • 鉴定出VEGF和TGFB1是内皮和视网膜色素上皮细胞中基因表达的潜在上游调节者.
  • 空间基因表达特征与之前的单细胞和小鼠模型研究进行了比较.

结论:

  • 这项研究提供了视网膜基因表达的空间地图,视网膜色素表皮和健康和MNV中的胸膜.
  • 确定了在MNV中失调的候选分子.
  • 这些发现提供了对MNV病变发生的细胞和分子机制的见解.