人类互白素-12α和EBI3是具有抗炎功能的细胞因子
Karen Hildenbrand1, Sina Bohnacker2, Priyanka Rajeev Menon1
1Center for Functional Protein Assemblies (CPA), Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, 85748 Garching, Germany.
Science advances
|October 25, 2023
概括
介素35 (IL-35) 子单元IL-12α和EBI3是稳定的抗炎分子. 它们独立减少炎症并促进调节性T细胞,提供新的治疗途径.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞因子研究 细胞因子研究
背景情况:
- 介质蛋白调节免疫反应;介质蛋白12 (IL-12) 家族在炎症和传染病中至关重要.
- 干白素35 (IL-35) 是IL-12家族的一种成员,在自身免疫性疾病和癌症中起着关键作用,但在分子水平上对它的了解很少.
研究的目的:
- 阐明IL-35,IL-12α和EBI3个别子单元的分子特征和功能作用.
- 调查IL-35子单元是否仅作为异构体而起作用,或者它们是否具有独立的生物活性.
主要方法:
- 分析IL-12α和EBI3的分泌模式.
- 评估孤立的IL-12α和EBI3蛋白质的稳定性和抗炎性质.
- 评估IL-12α和EBI3对促炎性细胞因子分泌和调节性T细胞发育的影响.
主要成果:
- IL-12α和EBI3相互促进它们的分泌,但可以彼此独立分泌.
- 作为单个蛋白质,IL-12α和EBI3都很稳定.
- 隔离的IL-12α和EBI3通过减少促炎性细胞因子分泌和诱导调节性T细胞,表现出抗炎作用.
结论:
- IL-35,IL-12α和EBI3的子单元是独立的功能性活跃的抗炎性免疫分子.
- 这一发现扩大了对人类细胞因子谱的理解,并为针对自身免疫性疾病和癌症的新型免疫治疗策略提供了基础.
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