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在子Tembusu病毒感染期间,NS5劫持TRAF3以抑制I型干扰素信号传递

Wenjun Cai¹, Yuhong Pan², Wanshuang Luo¹

¹Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan 611130, China.

Veterinary microbiology

|October 25, 2023

概括

瘤坏死因子 (TNF) 受体相关因子3 (TRAF3) 作为一种抗病毒蛋白. TRAF3的过度表达抑制了Tembusu病毒 (DTMUV) 的复制,并增强了对病毒入侵的先天免疫力.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

瘤亡因子 (TNF) 受体关联因子3 (TRAF3) 通过RIG-I通路在先天免疫中至关重要.
虽然已知哺乳动物TRAF3的功能,但其在子中的作用尚未被探索.

研究的目的:

为了克隆和表征TRAF3 (duTRAF3).
研究duTRAF3在子对病毒感染的天生的免疫反应中的作用,特别是子Tembusu病毒 (DTMUV).

主要方法:

克隆和测序马尔拉德的Anas platyrhynchosTRAF3基因.
对幼的duTRAF3组织分布的分析.
在对DTMUV感染的反应中研究duTRAF3的表达和功能.
评估DTMUV NS蛋白5与duTRAF3.3之间的相互作用.

主要成果:

duTRAF3与哺乳动物TRAF3共享功能领域.
duTRAF3在硬质腺,心脏和肺部表达高.
在DTMUV感染上,dUTRAF3的表达会升级.
过度表达duTRAF3抑制DTMUV复制并增强IFN-α和ISG转录.
DTMUV NS蛋白5通过抑制其表达来对抗duTRAF3.

结论:

duTRAF3作为抗病毒蛋白对抗DTMUV的功能.
duTRAF3在子对DTMUV.的天生的免疫防御中发挥着重要作用.
这些发现为开发新的反DTMUV策略提供了基础.

关键词:

在DTMUV NS5中使用.子的 TRAF3 天生的免疫系统天生的免疫系统.病毒复制是病毒的复制.

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