长非编码RNAMALAT1和HOTAIR表达与精子参数,DNA和男性不孕不育中malondialdehyde水平之间的关系
Afsaneh-Jaberi Asl1, Mohammadreza Sharifi2, Abolfazl Dashti3
1Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Tissue & cell
|October 25, 2023
概括
MALAT1和HOTAIR长非编码RNAs (lncRNAs) 的低表达与不育男性的精子运动性降低,DNA损伤增加和更高的甲酸 (MDA) 水平有关. 这些发现表明改善精子功能的潜在治疗点.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 精子质量对于评估男性生育能力至关重要.
- 长非编码RNAs (lncRNAs) MALAT1和HOTAIR在精子发育和氧化应激中发挥作用.
- 了解lncRNA表达对于评估男性不孕症至关重要.
研究的目的:
- 调查MALAT1和HOTAIR表达和精子参数之间的关联.
- 为了确定这些lncRNAs,精子DNA碎片化和马隆迪化 (MDA) 水平之间的关系.
- 探索MALAT1和HOTAIR作为男性生育能力生物标志物的潜力.
主要方法:
- 分析了30名肥沃和30名不育男性的精液样本.
- 用计算机辅助精子分析 (CASA) 来评估精子参数.
- 用Acridine Orange (AO) 试验评估精子DNA完整性,通过铁酸反应评估MDA水平,通过RT-PCR评估lncRNA表达.
主要成果:
- 不育的男性表现出明显较低的MALAT1和HOTAIR表达水平 (p < 0.001).
- 马拉特1和HOTAIR表达与精子运动性和形态学正相关 (p < 0.001).
- 减少的lncRNA表达与DNA损伤和MDA水平负相关 (p < 0.001).
结论:
- 马拉特1和HOTAIR的低表达与精子功能受损有关,包括精子运动率降低,DNA损伤增加和MDA水平升高.
- MALAT1和HOTAIR代表了提高精子质量和男性生育能力的潜在治疗点.
- 这项研究强调了 lncRNAs 在男性生殖健康中的重要性.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:23Infertility in Males
276
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
276
01:41Spermatogenesis
102.6K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.6K
03:18Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
01:22MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K