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开发针对瘤相关抗原乳头瘤病毒结合因子的T细胞受体工程T细胞

Shuto Hamada^1,2, Tomohide Tsukahara¹, Yuto Watanabe^1,2

¹Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.

|October 25, 2023

概括

乳头瘤病毒结合因子 (PBF) T细胞受体工程T细胞 (TCR-T细胞) 显示出对肉瘤免疫疗法的希望. 在临床前模型中,这些PBF TCR-T细胞有效地向并减少表达PBF的瘤.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
细胞疗法细胞疗法

背景情况:

乳头瘤病毒结合因子 (PBF) 是一种骨髓瘤抗原.
由PBF衍生的接种会引起强烈的免疫反应.
通过T细胞受体工程的T细胞 (TCR-T细胞) 提供了向免疫治疗方法.

研究的目的:

开发PBF特异性的TCR-T细胞,用于潜在的肉瘤免疫疗法.
在临床前模型中评估PBF TCR-T细胞的疗效.

主要方法:

来自健康捐赠者的生成PBF TCR-T细胞.
使用HLA-A*24:02/PBF胺四聚合物用于检测.
创建混合PBFTCR-T细胞用于采用细胞疗法.
在NSG老鼠中评估抗瘤效应,这些老鼠被移植了PBF表达肉瘤细胞.

主要成果:

PBF TCR-T细胞表现出对PBF脉冲细胞和PBF转移细胞的识别.
混合PBFTCR-T细胞对各种PBF结构表现出反应性.
混合PBFTCR-T细胞在体内表现出显著的抗瘤作用.
在异种移植的瘤中观察到CD8+ T细胞的透和活性.

结论:

PBF TCR-T细胞疗法是治疗高度表达PBF的瘤的潜在候选人.
该研究建立了PBF TCR-T细胞治疗的临床前模型.
对瘤的PBF向免疫疗法的进一步研究是有必要的.

关键词:

在HLA-A24中,它具有HLA-A24的特征.在PBBF的PBBF.这就是TCR-T.混合动力TCR的混合动力TCR.骨质肉瘤是骨质肉瘤的一种疾病.

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