高吞吐量qPCR揭示了热带废水和河水中的共享抗生素耐药性基因
Thitima Srathongneam1, Montakarn Sresung2, Phongsawat Paisantham2
1Program in Applied Biological Sciences, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
The Science of the total environment
|October 25, 2023
概括
抗生素耐药性基因 (ARG) 在泰国水系统中普遍存在,废水和河水之间没有显著差异. 季节性变化和不充分的治疗影响ARG水平,强调需要更好的控制措施.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 分子生物学分子生物学
背景情况:
- 抗菌素耐药性 (AMR) 的上升是全球健康的威胁.
- 一个健康的方法强调对抗生素耐药性基因 (ARG) 的环境监测.
- 泰国缺乏关于不同环境环境和不同季节的ARG流行情况的全面数据.
研究的目的:
- 调查泰国废水和河水中ARG的流行和分布.
- 确定在水生环境中流通的特定ARG和移动遗传元素.
- 评估废水处理和季节性变化的对ARG污染的影响.
主要方法:
- 高通量定量聚合酶连锁反应 (qPCR) 用于ARG分析.
- 水样包括流入的废水 (n=12),排放废水 (n=12) 和河水 (n=12).
- 分析的重点是ARG和移动遗传元素的相对丰度.
主要成果:
- 在所有水样品的87%中检测到ARG,在流入水,废水和河水中均存在.
- 像intI1,tnpA和intI3这样的基因非常丰富,这表明河流的废水污染.
- 几种ARG (例如,blaVIM,mcr-1,vanA) 在废水和河水中比流入水更为丰富,这表明处理效率低下.
- 在旱季与雨季相比,九种特定的ARG在河水中更为普遍.
结论:
- 泰国水生环境中ARG的广泛传播需要紧急干预.
- 污水处理厂可能不充分去除ARG,导致环境污染.
- 季节性因素影响ARG污染模式,强调需要适应性管理策略.
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