转化能力与Haemophilus influenzae中的抗菌素耐药性潜力之间的关联
Emi Tanaka1, Takeaki Wajima1, Ruri Ota1
1Department of Microbiology, Faculty of Pharmacy, Meijo University.
Biological & pharmaceutical bulletin
|October 25, 2023
概括
在Haemophilus influenzae中,横向基因转移增加了对多种抗微生物药物的耐药性,包括诺和β-乳酸盐. 这种增强的转化能力引发了人们对抗菌素耐药性传播的担忧.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在日本,类素低敏感性Hemophilus influenzae的流行率增加.
- 低类素易感性源于目标基因突变或通过自然转换的横向基因转移.
- 需要研究横向基因转移与对其他抗微生物药物的耐药性之间的联系.
研究的目的:
- 检查Hemophilus influenzae.中水平基因转移能力与对诺和β-乳酸盐的耐药性之间的关联.
- 用实验转移试验量化水平转移能力.
- 为了研究共操作子在DNA吸收中的作用.
主要方法:
- 实验转移试验量化50个临床分离的Hemophilus influenzae的水平转移能力.
- 研究转化效率与对β-乳酸盐的耐药性之间的关联.
- 在具有高转换效率的菌株中分析comA转录水平.
主要成果:
- 在隔离物中,转化效率差异很大 (10^2到10^6 CFU).
- 高水平转移能力与由于ftsI突变而导致的β-乳抗性有关.
- 具有高转化效率的菌株表现出增加的comA转录水平,表明增强了DNA吸收.
- 水平转移能力与多种抗菌素耐药性相关.
结论:
- 海莫菲勒斯流感的转化能力与多种抗菌素耐药性有关.
- 具有高水平转移能力的菌株的患病率增加有助于抗菌素耐药性的传播.
- 增强的共操作子活动有助于高DNA吸收能力.
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