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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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时间分辨率的中红外光热显微镜用于成像嵌入水中的子束

Panagis D Samolis1,2, Xuedong Zhu2,3, Michelle Y Sander1,2,3,4

  • 1Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts 02215, United States.

Analytical chemistry
|October 25, 2023
PubMed
概括

一种新的时间分辨率的中红外光热显微镜技术使得在水中无标签成像成为可能. 这种方法通过分析短暂的热力学来区分生物组织与背景.

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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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