相关实验视频

Updated: Jul 12, 2025

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Published on: May 14, 2021

酸功能化 Pyroptosis 纳米调器用于表观遗传调节和增强癌症免疫治疗

Jiahao Hu¹, Yuchan You¹, Luwen Zhu¹

¹Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yu-Hang-Tang Road, Hangzhou, 310058, China.

Small (Weinheim an der Bergstrasse, Germany)

|October 26, 2023

概括

这项研究开发了一种新型纳米药物,以克服癌症免疫治疗中低响应率. 这种纳米药物增强了 pyroptosis 和 T 细胞的透,显著提高了免疫检查点在瘤中的阻断效率.

科学领域:

在瘤学瘤学.
纳米医学是一种纳米医学.
免疫治疗是一种免疫疗法.

背景情况:

免疫检查点阻塞 (ICB) 疗法面临挑战,包括低响应率和非目标效应.
热,一种免疫细胞死亡 (ICD) 机制,可能会增强抗瘤免疫反应.
由于mRNA甲基化导致低Gasdermin-E (GSDME) 表达,限制了某些瘤中的热致死诱导,阻碍了ICB的疗效.

研究的目的:

开发一个有针对性的纳米系统来增强 pyroptosis 和提高 ICB 疗效在瘤低GSDME表达.
为了研究结合表观遗传改变与激光灭诱导用于癌症治疗的潜力.

主要方法:

用酸功能化的脂质体与迪西他 (脱甲基化剂) 和三班达 (火致死诱导剂) 共同加载.
纳米系统的瘤积累被评估使用酸和Siglec受体向.
评估了激素灭绝诱导,免疫细胞透 (CD8+ T细胞) 和抗瘤疗效.

主要成果:

这种纳米药物在瘤中增加了积 (24小时后增加了3.84倍).
观察到与烧死相关的蛋白质和caspase-3/GSDME依赖性烧死的升级.
CD8+ T 细胞在瘤微环境中显著透,并实现了89.1%的瘤抑制率.

结论:

酸功能化的 pyroptosis 纳米药物提供了一个有希望的策略,以克服瘤中低GSDME表达.
这种方法通过诱导免疫细胞死亡和促进T细胞介导的抗瘤免疫力来增强ICB疗法.
表观遗传修饰与热致死诱导相结合,代表了改善癌症治疗结果的可行策略.

关键词:

癌症免疫疗法免疫疗法组合疗法治疗组合治疗.表观遗传学是指表观遗传学.热致灭 (pyroptosis) 是一种致的过程.瘤向向向的目标

更多相关视频

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Published on: June 13, 2014

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...