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在Dicentrarchus labrax病毒的毒性中Aeromonas基因型定型的作用

Ibrahim Mohamed Aboyadak¹, Magdy Khalil Soliman², Hadeer Mohamed Nageeb²

¹National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.

Journal of fish diseases

|October 26, 2023

概括

欧洲海的空气菌败血症是水产养殖的一个主要问题. 这项研究确定了Aeromonas caviae和A. veronii,详细介绍了它们的毒性基因和有效的抗生素,为鱼类健康管理提供了洞察力.

科学领域:

水生动物的健康状况.
细菌学细菌学是一门学科.
鱼类免疫学鱼类免疫学

背景情况:

气菌的感染病症对全球水产养殖业构成重大威胁,特别是影响欧洲海 (Dicentrarchus labrax).
之前在欧洲海养殖场爆发的疫情与高死亡率有关.

研究的目的:

为了识别和描述导致欧洲海底的败血症的Aeromonas物种.
为了研究孤立的Aeromonas菌株的毒性因素和抗生素敏感性.
分析Aeromonas感染对鱼类生理压力标志物的影响.

主要方法:

从患病的鱼中分离和生物化学识别 (Vitek 2系统) 细菌病原体.
使用PCR和植物遗传学分析确认物种识别.
使用分子方法检测关键的毒性基因 (hlyA, aer, act, ast).
确定致命剂量50 (LD50) 以评估病原性.
对常见的抗微生物药物的抗生素敏感性测试.
在受感染的鱼中测量血清皮质醇,甲酸酶和谷氨过氧化酶水平.

主要成果:

从受影响的欧洲海养殖场成功分离出了Aeromonas caviae和Aeromonas veronii.
病毒性基因血清素 (hlyA) 和气清素 (aer) 在分离物中普遍存在.
在D. labrax的指纹中,Aeromonas caviae表现出比Aeromonas veronii更高的致病性 (较低的LD50).
诺夫洛克萨,多西环素和氧环素对测试的Aeromonas分离体显示出有效性.
感染导致血清皮质醇增加和抗氧化酶活性 (catalase,谷氨过氧化酶) 最初降低,随后恢复.

结论:

在Aeromonas分离物中病毒性基因的存在与鱼类死亡率和病原性增加直接相关.
在欧洲海中,Aeromonas感染引发了显著的生理应激反应,影响了应激激素和抗氧化剂防御.
已识别的抗生素为水产养殖环境中的Aeromonas败血症提供了可行的治疗选择.
了解毒性因素和宿主反应对于管理鱼类养殖中与Aeromonas相关的疾病至关重要.

关键词:

这是一个PCRPCR.皮质醇是一种皮质醇.引入引入引入引入移动的飞败血症败血症灵敏度灵敏度灵敏度灵敏度灵敏度测序的测序是指测序的测序.

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