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MALDI质谱成像和局部递送的乳酸的半量化

Aaron Cohen¹, Raphael Legouffe², Junhong Mao¹

¹Personal Care Department of the Colgate-Palmolive Company, Piscataway, New Jersey, USA.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

|October 26, 2023

概括

在皮肤护理中测量局部乳酸输送具有挑战性. 一种使用三同位素乳酸和MALDI-MSI的新方法成功地量化和确定其从各种产品中透到皮肤的质量.

科学领域:

皮肤病学皮肤病学
分析化学分析化学
生物化学生物化学

背景情况:

乳酸是局部护肤的关键成分.
由于高的内源水平,测量其皮肤输送是困难的.
需要新的方法来准确评估局部乳酸透率.

研究的目的:

开发一种定量和定性方法来测量乳酸进入皮肤的量度.
分析各种护肤配方的乳酸透率.
为了克服皮肤内源性乳酸所带来的挑战.

主要方法:

使用矩阵辅助的激光消耗/电离质谱成像 (MALDI-MSI).
为了信号分辨率,使用了三重乳酸同位素 (L-乳酸-13C3).
分析了用乳酸护肤产品处理的猪皮肤样本.

主要成果:

通过使用L-乳酸-13C3.3.成功地从内源背景信号中解决局部乳酸.
能够对皮肤部分的乳酸分布进行定量和定性分析.
证明了MALDI-MSI的有效性,用于分析各种产品的乳酸输送.

结论:

联合使用L-乳酸-13C3和MALDI-MSI提供了一种可靠的方法来测量局部乳酸输送.
这种方法准确地量化和合格乳酸透到皮肤.
未来的研究可以利用这种方法来阐明乳酸在护肤中的作用模式.

关键词:

弗朗茨的细胞是弗朗茨的细胞.阴阳性西烯的存在.化学剥皮是一种化学剥皮.衍生化的衍生化同位素标签的标签层层的角质层 (stratum corneum) 是一个层.三酸是三酸的一种.

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