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对酸性压力的反应机制促进了巨细胞中的LF82复制
Ting Yao1,2, Yu Huang1,2, Zimeng Huai1,2
1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China.
Frontiers in cellular and infection microbiology
|October 26, 2023
概括
粘附侵入性大肠杆菌 (AIEC) LF82通过激活酸性冲击基因和增强酸盐使用,在巨细胞中复制. 酸性条件和酸盐会促进AIEC LF82的复制,而鞭毛基因则被降低调控,从而减少运动性和入侵性.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 粘附性侵入性大肠杆菌 (AIEC) LF82在肠道上皮细胞和巨细胞中表现出粘附性,入侵性和细胞内复制性.
- AIEC LF82可以在巨细胞内复制而不诱导宿主细胞死亡,突出其生存机制.
研究的目的:
- 在酸性条件下研究AIEC LF82在巨细胞内复制的转录和遗传基础.
- 阐明酸性冲击基因,酸盐代谢和鞭毛功能在AIEC LF82病变发生过程中的作用.
主要方法:
- RNA测序和qRT-PCR用于在不同pH值下分析LF82中的差异表达基因 (DEGs).
- 构建DEGs的删除突变,并分析它们的复制,粘附和入侵能力.
- 在各种条件下测试鞭毛运动和巨细胞复制,包括酸中和酸补充剂.
主要成果:
- 细胞内酸化对于巨细胞内的AIEC LF82复制至关重要.
- 酸性冲击和酸性适应基因的升级促进了LF82的复制;酸盐治疗进一步增强了复制.
- 代谢基因缺失 (ΔnarK, ΔnarXL, ΔnarP, Δhmp) 降低了LF82复制;鞭毛基因下调 (ΔfliC, ΔfliD) 损害了运动性,粘附性和入侵性,但没有复制.
结论:
- AIEC LF82通过激活酸性冲击/健身系统并增强酸利用,促进巨细胞的复制.
- 酸性环境和酸盐的可用性是影响LF82细胞内生存和增殖的关键因素.
- 在酸性条件下抑制鞭毛功能有助于减少移动性和宿主细胞相互作用.
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