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在THP-1单细胞对促炎性巨细胞的两极化过程中,GABAergic和purinergic成分表达的动态变化发生

Victor Manuel Ruiz-Rodríguez¹, Carlos Alberto Torres-González^1,2, Karina Monserrat Salas-Canedo^1,2

¹División de Biología Molecular, Laboratorio de Neurobiología, Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), San Luis Potosí, San Luis Potosí, México.

Biochemistry and biophysics reports

|October 26, 2023

概括

单细胞和巨细胞在激活和分化过程中动态地改变它们的GABAergic和purinergic信号元件的表达. 这些变化表明,在炎症中,神经系统和免疫系统之间存在一种新的相互作用途径.

科学领域:

免疫学免疫学免疫学
神经科学是一个神经科学.
细胞生物学细胞生物学

背景情况:

单细胞是关键的先天性免疫细胞,分化为具有多种表型的巨细胞.
胺黄油酸 (GABA) 和氨酸三酸 (ATP) 是影响单细胞分化和巨细胞两极化的神经递质.
在单细胞-巨细胞过渡期间,GABAergic和purinergic系统的表达动态在很大程度上仍未被描述.

研究的目的:

为了研究THP-1单细胞中GABAergic和purinergic信号组件的表达水平和变化.
分析这些变化在单细胞激活,分化和两极化到M1亲炎性巨细胞.

主要方法:

在实验研究中利用了THP-1单细胞细胞系.
评估了关键GABAergic (GABA载体2,谷氨酸脱酶-67,GABA A型受体子单元) 和纯能 (P2X受体) 组件的表达.
在单细胞激活,分化和M1极化期间监测表达变化.

主要成果:

激活的单细胞显示GABA载体2和谷氨酸脱酶-67的增加,以及明显的P2X4和P2X7受体表达.
单细胞分化导致GABA A型受体β2亚单元的表达增加,P2X1和P2X1del的减少.
预炎性M1巨体表现出减少的GABA A型受体α4亚单元和谷氨酸脱酶-67,但过度表达的P2X4和P2X7.

结论:

从单细胞转变为巨细胞的转变涉及GABAergic和purinergic信号组件的显著动态变化.
单细胞和巨细胞对神经递质刺激做出反应,表明在炎症中可能存在神经免疫系统相互作用.
这些发现提供了关于神经和免疫系统在外周和神经炎症中的相互作用的见解.

关键词:

对于GABA来说,这是一个很好的选择.在GABA-AR组合中.在GAD67中,GAD67是最重要的.在M1巨细胞中.这是一个P2X1P2X1P.在P2X1del中,我们可以使用P2X1del.这是一个P2X4P2X4P.

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