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对四种工程化PET降解酶的评估,考虑到大规模的工业应用

Grégory Arnal¹, Julien Anglade², Sabine Gavalda¹

¹Carbios, Parc Cataroux-Bâtiment B80, 8 Rue de la Grolière, 63100 Clermont-Ferrand, France.

ACS catalysis

|October 26, 2023

概括

聚乙烯二甲 (PET) 的酶循环利用通过标准化水解协议得到了推进. LCC^ICCG酶表现出卓越的性能,实现了98%的PET脱聚合,并为工业扩展提供了最佳条件.

科学领域:

生物技术是生物技术.
聚合物科学聚合物科学
环境科学环境科学

背景情况:

酶回收为聚乙烯二甲 (PET) 废物提供了一个可持续的替代品,特别是对于具有挑战性的材料.
蛋白质工程已经产生了大量的PET降解水溶酶,但不一致的实验方法阻碍了性能比较.
标准化协议对于评估和扩大酶性PET水解至关重要.

研究的目的:

为了建立一个标准化的酶化水解协议的聚乙烯二甲 (PET) 相关的工业规模.
在标准化条件下对四种突出的PET水解酶 (LCC,ICCG,FAST-PETase,HotPETase和PES-H1) 的性能进行比较评估.
为最有前途的酶优化反应条件,以提高经济可行性.

主要方法:

为PET开发一个标准化的酶性水解协议.
该协议的应用用于评估LCC,ICCG,FAST-PETase,HotPETase和PES-H1.
对LCC^ICCG的酶负载和反应温度的优化.

主要成果:

由于低脱聚合率,FAST-PETase和HotPETase在大规模应用方面存在局限性.
PES-H1^L92F/Q94Y实现了80%的PET脱聚合,表明了随着进一步发展而产业扩展的潜力.
LCC^ICCG表现出卓越的性能,在24小时内实现了98%的PET去聚合,转化为甲基甲酸 (TPA) 和乙烯基醇 (EG).
针对LCC^ICCG的优化条件使酶使用量减少了3倍,反应温度从72°C降低到68°C.

结论:

一个标准化的协议有助于对PET水溶酶进行一致的评估和比较.
LCC^ICCG是一种非常有前途的酶,用于工业规模的酶性PET回收利用.
对LCC^ICCG的优化条件提高了其经济可行性,以便大规模实施.

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