一种新的方法用于自动化晶体可视化和量化在小鼠叶酸诱导的急性损伤
Ahmad Kamal Hamid1,2, Eva Maria Pastor Arroyo1,2, Sung Sik Lee3,4
1Institute of Physiology, University of Zurich, Zurich, Switzerland.
American journal of physiology. Renal physiology
|October 26, 2023
概括
我们开发了一种新的组织学方法,用于在动物急性损伤 (AKI) 模型中可视化和量化叶酸 (FA) 晶体. 这项技术揭示了FA-AKI是性别依赖的,女性表现出更高的晶体负担和改变的FGF23水平.
科学领域:
- 脏病学和泌尿病学 医学
- 组织病理学和成像学
- 疾病的动物模型.
背景情况:
- 叶酸 (FA) 诱导的急性损伤 (FA-AKI) 是一种常见的动物模型.
- 现有关于FA-AKI的文献受限于缺乏可视化和量化脏FA晶体的方法.
- 准确的量化对于理解FA-AKI机制和开发干预措施至关重要.
研究的目的:
- 为FA晶体可视化和量化开发一种简单的,保存晶体的组织学协议.
- 为了自动化成像和分析,以高效地测量晶体含量.
- 使用开发的方法在雄性和雌性小鼠中描述FA-AKI模型.
主要方法:
- 使用标准实验室材料开发了一种晶体保存组织学协议.
- 结合组织学与自动成像用于FA晶体可视化.
- 创建了一个自动化宏来量化脏FA晶体含量.
主要成果:
- 该方法成功地可视化和量化了皮层和骨髓区域的FA晶体.
- 与雄性相比,雌性小鼠在FA注射后30小时显示出明显更高的脏FA晶体含量,脏重量和血尿素.
- 雌性表现出高血完整纤维细胞生长因子23 (FGF23) 和骨 *Fgf23* mRNA表达,尽管血酸盐水平相似.
结论:
- 这种新方法在FA-AKI模型中为量化脏FA晶体提供了有价值的工具.
- FA-AKI模型显示了结晶沉积和相关病理的性别差异.
- 这种技术有助于FA-AKI的机制研究和治疗干预研究中的结晶变异性控制.
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