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沉默Lnc-HES1-10 抑制骨髓瘤细胞的增殖,侵入能力和转移

Yingchun Zhu¹, Chi Zhang, Caihua Zhang

¹Department of Orthopedic Surgery, Ningbo First Hospital, Ningbo, China.

Journal of pediatric hematology/oncology

|October 26, 2023

概括

长非编码RNA HES1-10 (Lnc-HES1-10) 在骨髓瘤 (OS) 中过度表达,促进瘤生长和转移. 沉默Lnc-HES1-10抑制了OS细胞的增殖,迁移和入侵,这表明它的关键作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNAs (LncRNAs) 与骨髓瘤 (OS) 的发展和病理学有关.
在OS中由HES1-10激活的LncRNAs的特定作用仍未得到充分研究.

研究的目的:

为了研究Lnc-HES1-10在骨髓瘤中的功能.
确定Lnc-HES1-10对OS细胞增殖,迁移和入侵的影响.

主要方法:

使用MTT测定评估的细胞增殖.
通过伤口愈合和Transwell测试评估的迁移和入侵.
定量逆转录酶聚合酶连锁反应和用于确定HES1-10表达水平的西部斑.

主要成果:

发现Lnc-HES1-10在OS组织中过度表达,并与患者预后不佳有关.
在MG63和143BOS细胞系中Lnc-HES1-10的过度表达促进了体外增殖.
沉默Lnc-HES1-10显著抑制了MG63和143B细胞的迁移和入侵能力.

结论:

Lnc-HES1-10在调节骨髓瘤细胞生长和转移方面发挥着至关重要的作用.
准Lnc-HES1-10可能代表骨髓瘤的潜在治疗策略.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...