长期储存不会影响玻璃化加热的人类胚胎的DNA甲基化概况
Ling Zhu1,2, Liwei Sun1,2, Weiwei Liu1,2
1Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Women and Children's Hospital of Chongqing Medical University, Chongqing, China.
Molecular reproduction and development
|October 26, 2023
概括
长期冷保存人类胚胎3年或8年不会改变单细胞水平的DNA甲基化概况. 这一发现支持了胚胎存储用于辅助生殖技术的安全性.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 辅助生殖技术 辅助生殖技术
背景情况:
- 胚胎冷保存对于辅助生殖技术至关重要.
- 长期储存对人类胚胎DNA甲基化的影响尚不清楚.
研究的目的:
- 研究冷保存时间对人类八细胞胚胎的DNA甲基化概况的影响.
- 评估长期存储胚胎的安全性.
主要方法:
- 单细胞全基因组二硫酸盐测序 (scWGBS) 在新鲜和冷保存的人类胚胎上进行.
- 胚胎被分为新鲜,冷保存3年和冷保存8年的样本.
- 分析了全基因组甲基化模式和印制基因甲基化.
主要成果:
- 在新鲜和冷保存的胚胎组中,全基因组甲基化模式相似.
- 印制基因中没有发现甲基化水平的显著差异.
- 鉴定出差异甲基化区域 (DMRs) 没有富含促进体,并且显示出类似的全基因组分布.
结论:
- 长期冷保存 (3年和8年) 在单细胞水平上不会显著影响人类八细胞胚胎的DNA甲基化概况.
- 观察到的DMR不太可能影响基因表达.
- 对于人类胚胎中的DNA甲基化完整性,冷保存的持续时间是安全的.
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