相关实验视频
Updated: Jul 12, 2025

06:16
mirMachine: A One-Stop Shop for Plant miRNA Annotation
Published on: May 1, 2021
2.6K
在MIR444c基因中编码的PEP444c调节了小麦中的microRNA444c积累
Aleksandra Chojnacka1, Aleksandra Smoczynska1, Dawid Bielewicz1,2
1Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland.
Physiologia plantarum
|October 26, 2023
概括
大麦MIR444基因产生具有开放的读取框架的primi-microRNAs444,这些基因被转化为蛋白质. PEP444c蛋白对于大麦植物中的微RNA444c生物发生是至关重要的.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 微RNAs (miRNAs) 是小型的,非编码的RNAs调节基因表达.
- MIR444基因家族是独一无二的,miRNAs444针对大米和大麦中的MADS盒转录因子.
- 研究了大麦MIR444基因 (MIR444a/b/c) 和它们的主要转录 (pri-miRNAs444).
研究的目的:
- 描述大麦primiRNAs的结构和加工444.4.
- 为了研究primi-miRNAs444.4的潜在蛋白质编码能力.
- 确定编码蛋白在miRNA生物发生中的作用.
主要方法:
- 大麦MIR444基因的识别和结构特征.
- 对pri-miRNA444替代拼接的分析.
- 对pri-miRNA转录的核糖体关联测定.
- 使用特定抗体对PEP444a和PEP444c蛋白质的免疫检测.
- 在CRISPR-Cas9中介的PEP444c编码序列的突变发生.
主要成果:
- 大麦primiRNAs444表现出广泛的替代拼接,产生功能和非功能异型.
- 在pri-miRNAs444转录中,多个开放的读取框架 (ORF) 与核糖体有关.
- 在大麦植物中表达PEP444a和PEP444c蛋白.
- 在大麦中,PEP444c编码序列的突变降低了PEP444转录水平和成熟的microRNA444c水平.
结论:
- 大麦的primiRNAs444通过ORFs具有蛋白质编码潜力.
- PEP444c蛋白在大麦中的microRNA444c的生物发生中发挥作用.
- 这项研究揭示了miRNA生物发生的新型调节机制,涉及miRNA基因的蛋白质产物.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Regulation of Expression Occurs at Multiple Steps
22.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.7K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K

