在应激适应中TRDMT1/DNMT2的作用及其在Physcomitrium patens中在透应激下对转录组和蛋白质组动态的影响
Nikita Wadhwa1, Darshika Singh1, Radha Yadav1
1University School of Biotechnology, Guru Gobind Singh Indraprastha University, Dwarka, Delhi, India.
Physiologia plantarum
|October 26, 2023
概括
tRNA甲基转移酶TRDMT1/DNMT2对于的透应激耐受性至关重要. 这种酶的损失会破坏参与新陈代谢和光合作用的基因网络,影响干旱条件下的植物生存.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 像Physcomitrium patens这样的早期陆地植物缺乏在气管细胞中发现的保护性结构.
- 这些植物依靠生理和生化适应来承受陆地压力,例如干旱.
- 对tRNA (cytosine(38) -C(5)) -甲基转移酶TRDMT1/DNMT2的功能丧失突变对氧化和透应激具有敏感性.
研究的目的:
- 研究PpTRDMT1/PpDNMT2在透应激下调节遗传网络中的作用.
- 了解P. patens.中透应激耐受性的基础分子机制.
主要方法:
- 在透应激下在野生类型和ppdnmt2突变植物中进行全基因组转录组和蛋白质组分析.
- 针对反应性氧物种进行血清化学染色和用于质细胞超结构的传输电子显微镜.
- 免疫沉结合质谱和酵母两杂交试验,以确定蛋白质相互作用.
主要成果:
- 在ppdnmt2突变体中发现了375个差异表达的基因,主要涉及碳水化合物代谢,光合作用和细胞生长.
- 在ppdnmt2突变体中观察到高反应性氧物种水平.
- 糖解路的蛋白质,抗氧化酶和与光合作用相关的蛋白质与PpTRDMT1/PpDNMT2共同净化,用甘甲基3-酸盐脱酶显示直接结合.
结论:
- PpTRDMT1/PpDNMT2通过影响能量代谢,表观遗传过程和转录,在透性应激耐受性中发挥着重要作用.
- 这项研究揭示了表观遗传修饰,能量代谢和植物应激反应中的转录调节之间的联系.
- 了解这些网络为提高早期陆地植物的应激弹性提供了洞察力.
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