PGAM5降解PDCoV N蛋白,并激活I型干扰素以对抗病毒复制
Xinyu Yang1, Ning Kong1,2, Wenzhen Qin1
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Shanghai, China.
Journal of virology
|October 26, 2023
概括
猪三角型冠状病毒 (PDCoV) 感染降低了PGAM5水平,有助于病毒传播. 然而,PGAM5触发了自并激活了免疫信号,以抑制小猪的PDCoV复制.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 猪三角冠状病毒 (PDCoV) 是一种三角冠状病毒,在小猪中引起显著的腹,发病率和死亡率.
- 自2012年发现后,PDCoV已经在全球范围内传播,对猪健康构成持续威胁.
- 病毒感染涉及病原体逃避策略和宿主防御机制之间的复杂相互作用.
研究的目的:
- 阐明PGAM5在宿主对PDCoV感染的反应中的作用.
- 研究PGAM5影响PDCoV复制的分子机制.
- 为了确定控制PDCoV爆发的潜在治疗目标.
主要方法:
- 研究了PDCoV感染期间PGAM5的表达水平.
- 利用共免疫沉和西式涂抹来分析蛋白质相互作用.
- 评估了PGAM5对病毒复制和宿主免疫路径的影响,包括自和IFN信号传递.
主要成果:
- 发现PDCoV感染降低了PGAM5表达的调节,促进了病毒复制.
- 通过与P62和STUB1.1的相互作用,PGAM5通过自性介导PDCoV核体 (N) 蛋白的降解.
- 通过与MyD88和TRAF3相互作用,PGAM5激活干扰素 (IFN) 信号通路,从而抑制病毒复制.
结论:
- 在PDCoV感染中,PGAM5扮演着双重角色:其下调调节促进病毒复制,而其功能活性抑制病毒.
- 这些发现突出了PGAM5介导的自和IFN信号通路作为对PDCoV的关键宿主防御机制.
- 准PGAM5通路可能为管理猪群中PDCoV提供一种新的策略.
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