PRMT5通过控制Ganoderma lucidum中陶马丁类蛋白质的剪接来调节多糖含量
Rui Liu1, Zhengyan Yang1, Tao Yang1
1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture; Microbiology Department, College of Life Sciences, Nanjing Agricultural University , Nanjing, China.
Microbiology spectrum
|October 26, 2023
概括
蛋白质氨酸甲基转移酶5 (PRMT5) 通过控制TLP替代拼接来调节Ganoderma lucidum的多糖生物合成. 这项研究揭示了PRMT5
科学领域:
- 菌类学和生物化学 菌类学和生物化学
- 分子生物学和遗传学
背景情况:
- 蛋白质氨酸甲基转移酶5 (PRMT5) 与Ganoderma lucidum中的二次代谢物生物合成有关.
- 在调节二次代谢产物的过程中,PRMT5的精确分子机制尚未完全被理解.
研究的目的:
- 为了阐明PRMT5在Ganoderma lucidum多糖生物合成中的作用.
- 研究PRMT5影响二次代谢产生的机制,特别关注替代拼接.
主要方法:
- 在Ganoderma lucidum中抑制PRMT5的基因沉默.
- 对多糖生物合成水平的分析.
- 研究TLP替代拼接及其异型.
- 评估与PGI结合的TLP2异型及其监管效应.
主要成果:
- 在G. lucidum中,PRMT5沉默显著降低了多糖类生物合成.
- 这种减少是由TLP基因的替代拼接介导的.
- 确定了一种特定的TLP2异型,直接与PGI结合,调节多糖类生物合成.
- PRMT5通过TLP具有约束力的监管来增强PGI活动.
结论:
- 通过TLP替代拼接,PRMT5在Ganoderma lucidum多糖生物合成中发挥着至关重要的作用.
- 这种TLP2异型是PRMT5-PGI相互作用途径的关键调解者.
- 这项研究确定了一种新的PRMT5标基因,并提供了对PRMT5调控机制的洞察.
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