PRMT5调解了FoxO1甲基化和亚细胞局部化,以调节肌原性祖先的脂质
Kun Ho Kim1, Stephanie N Oprescu2, Madigan M Snyder2
1Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
Cell reports
|October 26, 2023
概括
蛋白质氨酸甲基转移酶5 (PRMT5) 对于正常的肌肉发育和再生至关重要. 缺少它会导致肌肉缩和卫星细胞功能受损,因为它会影响FoxO1的稳定性和脂质滴滴代谢.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 肌肉生物学 肌肉生物学
背景情况:
- 蛋白质甲基化是细胞过程的关键调节者.
- 蛋白质氨酸甲基转移酶5 (PRMT5) 与瘤发生有关,但其在正常发育中的作用尚不清楚.
- 肌体发生,肌肉组织的发展,需要精确调节细胞增殖和分化.
研究的目的:
- 研究PRMT5在肌肉发育和肌肉发育中的作用.
- 阐明PRMT5调节肌肉形成和再生的分子机制.
主要方法:
- 使用Myod1Cre生成一个有条件的淘汰赛小鼠模型 (Prmt5MKO),以在胚胎髓细胞中删除Prmt5.
- 在Prmt5MKO小鼠中分析肌肉形态,肌细胞增殖,分化和卫星细胞功能.
- 研究受PRMT5缺乏影响的分子标和途径,包括FoxO1,自和脂质滴.
主要成果:
- Prmt5MKO小鼠表现出正常的出生,但发展渐进的肌肉缩和过早死亡.
- 失去PRMT5会抑制肌细胞增殖,促进过早分化,并损害卫星细胞的再生能力.
- 缺乏PRMT5导致FoxO1总水平的增加,细胞质积累,自的激活,以及脂质滴滴的枯竭.
- 抑制自部分挽救脂质滴并改善Prmt5MKO小鼠的肌肉再生.
结论:
- PRMT5对于正确的肌肉发育和再生至关重要.
- PRMT5至少部分通过调解FoxO1甲基化来调节肌体发生,从而影响其稳定性和局部化.
- 根据PRMT5对FoxO1的调节会影响自和脂质滴滴循环,这是肌肉平衡的关键过程.
关键词:
CP: 发育生物学科普:分子生物学 分子生物学在PRMTMT中使用.在PTM的PTM.这是SCs,SCs.自自是自的过程.肌肉发育 (myogenesis) 是一个过程.后翻译修改后的修改.蛋白质氨酸甲基转移酶是一种蛋白质氨酸甲基转移酶.卫星细胞 卫星细胞卫星细胞更多相关视频
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