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相关概念视频

CRISPR01:59

CRISPR

52.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

23
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
23
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K

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相关实验视频

Updated: Jul 12, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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多复合的基于CRISPR的核酸检测使用单个案例蛋白质.

Jinyu Fu1, Linghao Zhang1, Yanlin Long1

  • 1Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

Analytical chemistry
|October 26, 2023
PubMed
概括

这项研究介绍了CRISPR-TMSD,这是一种使用单个Cas12a蛋白用于敏感核酸检测的新型多重CRISPR诊断系统. 它实现了单核酸分辨率和高灵敏度,克服了以前的多重复合限制.

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 诊断检测试验 诊断检测试验

背景情况:

  • 基于CRISPR的核酸检测为分子诊断提供了方便,快速和灵敏度.
  • 目前的CRISPR系统的一个主要局限性是缺乏多重复合能力,通常需要多个Cas蛋白.

研究的目的:

  • 开发基于CRISPR的多重核酸检测系统,使用单个Cas蛋白进行单核酸分辨率.
  • 克服现有的CRISPR诊断平台的多重复合限制.

主要方法:

  • 开发了CRISPR-TMSD,将脚介导的链位移 (TMSD) 与Cas12a的 cis-cleavage活动相结合.
  • 使用针对特定目标的报道器和用于TMSD报道器设计的计算模拟工具包.
  • 结合CRISPR-TMSD与复合酶聚合酶放大 (RPA) 来提高检测灵敏度.

主要成果:

  • 使用单个Cas蛋白 (Cas12a) 实现了多重检测,具有单核酸分辨率.
  • 在与RPA结合时,已证明检测极限低至1副本/μL.
  • 成功检测出致癌基因和SARS-CoV-2RNA,验证了系统的性能.

结论:

  • CRISPR-TMSD提出了基于CRISPR的诊断系统设计的新策略,使单个Cas蛋白能够进行多重复合.
  • 该系统提供高灵敏度,特异性和单核酸分辨率,扩大了CRISPR的诊断应用.
  • 这种方法在推进分子诊断和疾病检测方面具有重大潜力.