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一种灵活的渐变横流免疫染色学试验,用于定性,半定量和定量测定血清粉样蛋白A

Guangtian Yang¹, Jishun Li¹, Shenglan Zhang¹

¹Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.

Journal of immunological methods

|October 26, 2023

概括

一个新的渐变侧流免疫测试试卷可以快速检测血清粉样蛋白A (SAA),这是一个重要的炎症标记. 这种可适应的条带提供了高精度的定性,半定量和定量SAA测量.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
免疫技术是一种免疫技术.

背景情况:

血清粉样蛋白A (SAA) 是一种关键的急性阶段蛋白质,表明炎症,感染或创伤.
准确及时检测SAA对于疾病诊断和监测至关重要.
现有的方法可能缺乏满足各种临床需求所需的灵活性或灵活性.

研究的目的:

开发一种用于SAA检测的新型渐变侧流免疫试验 (LFIA) 试验条.
为了使用单一的测试带进行定性,半定量和定量SAA测量.
为SAA分析提供灵敏,准确和适应性的工具.

主要方法:

开发了一个渐变LFIA测试带,使用金纳米粒子作为信号记者.
采用了三条测试线 (T1,T2,T3) 以降低抗体度用于差异SAA检测.
利用定性视觉检查,半定量线数,以及基于智能手机/扫描仪的定量图像分析来读取结果.

主要成果:

渐变的LFIA条纹显示出高相关性 (R2 = 0.998) 对于从5到1000μg/mL的定量SAA检测.
达到的检测极限低至9.33 ng/mL (智能手机) 和3.06 ng/mL (扫描仪).
结果显示与商业SAA测试条的良好一致,验证了其在人类血清样本上的性能.

结论:

开发的梯度LFIA测试条为SAA评估提供了一个多功能平台.
它提供可靠的定性,半定量和定量SAA测量.
这项技术为在各种临床环境中对SAA监测提供了灵活和适应性的解决方案.

关键词:

渐变侧流免疫测试试卷的测试带.定量分析是一种量化分析.血清中的粉样蛋白A.

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