转录组分析显示,在米的OsRpp30介导的疾病耐药性中,有IncRNA-miRNA-mRNA调节网络
Minghua Li1, Wei Li2, Meixia Zhao3
1Department of Biology, Miami University, Oxford, OH, 45056, USA.
BMC genomics
|October 26, 2023
概括
长非编码RNAs (lncRNAs) 对大米免疫非常重要. 这项研究揭示了 lncRNAs.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 基因组学和转录基因组学
背景情况:
- 长非编码RNAs (lncRNAs) 是植物生物过程的组成部分,包括疾病耐药性.
- 米树的RNase P蛋白30 (OsRpp30) 是已知的对抗病原体的米免疫力的积极调节者.
- 在OsRpp30介导的病耐药性中,lncRNAs的具体作用尚不清楚.
研究的目的:
- 综合分析不同OsRpp30水平 (野生类型,过度表达和淘汰赛) 的米植物中的lncRNA,miRNA和mRNA的表达模式.
- 调查lncRNAs和mRNAs之间的调控相互作用,包括跨和cis向.
- 构建一个竞争的内源RNA (ceRNA) 网络,以阐明lncRNAs,miRNAs和mRNAs在米病耐药性的相互作用.
主要方法:
- 使用高通量RNA测序,对野生类型,OsRpp30-OE和OsRpp30-KO大米系中的lncRNA,miRNA和mRNA表达进行了分析.
- 进行生物信息分析以识别差异表达基因 (DEG) 和预测lncRNA-mRNA相互作用 (跨和 cis向).
- 一个ceRNA网络被构建以整合lncRNA,miRNA和mRNA调节关系.
主要成果:
- 在分析的米系中,鉴定出91个差异表达的lncRNAs (DElncRNAs),1671个DEmRNAs和41个DEmiRNAs.
- 发现了10个跨向的lncRNA-mRNA对和27个向的cisRNA对.
- 构建一个ceRNA网络,揭示了参与病原体识别,激素信号和转录因子活性的基因的lncRNA和miRNA调节,这对植物免疫至关重要.
结论:
- 这项研究提供了米防突变中的lncRNAs,miRNAs和mRNAs的综合转录基因概况.
- 构建的跨向和 cis向网络,以及 ceRNA 网络,为 lncRNAs 的功能角色提供了洞察力.
- 这些发现强烈支持lncRNAs在米中OsRpp30介导的疾病耐药性的显著参与.
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