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在亚抑制度的抗微生物酶lysostaphin调节葡萄球菌粘附,生物膜形成和毒素生产

Yuan Yue^1,2, Ke Chen^1,3, Changfeng Sun^1,3

¹Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.

BMC microbiology

|October 26, 2023

概括

抗微生物酶lysostaphin (APLss) 有效地抑制葡萄球菌对宿主细胞的粘附,并减少生物膜的形成. 这种抗病毒性策略在通过向葡萄球菌毒性因子来对抗抗菌素耐药性方面表现有前途.

科学领域:

微生物学微生物学
生物化学生物化学
药物发现药物发现药物发现

背景情况:

抗微生物耐药性是一种全球性威胁.
抗微生物药物可以用作抗病毒策略.
感染部位的抗生素度低于最佳值令人担忧.

研究的目的:

为了检查抗微生物酶溶菌素 (APLss) 对葡萄球菌粘附的作用.
调查APLss对生物膜形成和毒素产生的影响.
探索APLss作为一种减轻葡萄球菌毒性的方法.

主要方法:

在大肠杆菌中的His标记成熟APLss的可溶性表达和净化.
对抗葡萄球菌菌株的最小抑制度 (MIC) 的确定.
超结构分析,光谱测量和光显微镜以评估粘附和生物膜形成.
病毒性基因的转录分析.

主要成果:

产生了高度纯净的APLss,并显示出对金黄色菌株 (MICs 1-2μg/mL) 的强有力的抑制作用.
APLss诱导了葡萄球菌表面膜的度特定变化.
APLss显著抑制了葡萄球菌对人类阴道上皮细胞的粘附和生物膜生物质的形成.
APLss抑制了定数感应效应器agrA和其他与毒素合成相关的毒性基因的表达.

结论:

APLss剂量依赖地抑制了宿主细胞粘附和葡萄球菌毒性因子.
APLss显示出作为一种具有抗粘合机制的抗葡萄球菌剂的潜力.
需要进一步在体内使用葡萄球菌毒性休克综合征模型进行体内调查.

关键词:

在APLssss中使用.生物膜抑制抑制的作用细胞粘附细胞粘附稳定溶解活性稳定溶解活性转录的变化是转录的变化.

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