一种新型的六次性NADP+降解[FeFe]酶来自Moorella thermoacetica
Florian P Rosenbaum1, Volker Müller1
1Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
这项研究确定了Moorella thermoacetica中的HydABCDEF酶,这是利用减少NADP+的关键酶. 这一发现对于通过伍德-Ljungdahl路径了解二氧化碳固定至关重要.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 像Moorella thermoacetica这样的乙菌利用木-Ljungdahl路径 (WLP) 来减少二氧化碳.
- WLP酶需要特定的减少剂,包括NADH,NADPH和减少的铁素.
- 虽然已知一种酶 (HydABC) 能降低铁素和NAD+,但降低NADP+的酶仍未确定.
研究的目的:
- 在Moorella thermoacetica中识别和描述负责NADP+减少的酶.
- 调查HydABCDEF酶在WLP和替代电子受体的背景下的作用.
主要方法:
- 从葡萄糖和DMSO上种植的Moorella thermoacetica中净化酶.
- 纯化酶的生物化学表征,包括子单元组成,辅因子分析和基质特异性.
- 用各种电子捐赠者和受体进行酶活性测定,包括H2和NADP+.
- 在不同的生长条件下对基因表达模式的分析.
主要成果:
- 六个子单位的HydABCDEF酶被净化,发现含有铁和FMN.
- 该酶特别减少了NADP +,并将H2作为减少剂,在60°C和pH 8时具有最佳活性.
- 对于这种酶,没有观察到与氨酸核酸和铁素的电子分支.
- 细胞悬浮体证明了依赖于H2的DMSO降解,支持HydABCDEF在H2到DMSO电子转移中的作用.
结论:
- 鉴定出HydABCDEF酶是M. thermoacetica中依赖H2的NADP+减少的主要酶.
- 这种酶可能在从H2到DMSO的电子转移中发挥作用,有助于生物体的代谢灵活性.
- 这些发现有助于更好地理解Wood-Ljungdahl路径内的减电剂再生和电子流.
更多相关视频
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10:10Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
Published on: December 1, 2020
相关概念视频
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Role of Reduced Coenzymes NADH and FADH₂
Electron Transport Chain: Complex III and IV
Electron Carriers
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Redox Reactions
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)