简单的方法来量化水溶液中的阳离子生物表面活性剂
Gabriele Sass1, Marie-Christine Groleau2, Eric Déziel2
1California Institute for Medical Research, San Jose, CA, United States.
Frontiers in bioengineering and biotechnology
|October 27, 2023
概括
我们开发了一种简单的纸质测试方法,通过测量其表面张力减少来量化生物表面活性剂. 这种方法可以准确地检测水溶液和细菌培养中的各种离子微生物表面活性剂.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 生物活性剂是多功能微生物化合物,在清洁,乳化和分散方面具有应用.
- 目前生物表面活性剂的检测和量化方法包括色度测试,HPLC和表面张力测量.
- 需要一种简单,易于使用的生物表面活性剂量化方法.
研究的目的:
- 介绍一种新的,简单的基于纸张的测定方法,用于量化生物表面活性剂.
- 用各种离子微生物表面活性剂验证试验.
- 为了证明该试验在监测细菌培养中的生物表面活性剂生产中的实用性.
主要方法:
- 一种基于其降低纸张表面张力的能力来量化生物表面活性剂的新方法,导致滴滴在油表面分散.
- 验证使用拉姆诺脂类,表面素,索法脂类和B.安纳托化物.
- 对*Pseudomonas aeruginosa*和*Burkholderia thailandensis*的培养物进行测试,包括相关突变物.
主要成果:
- 该试验表明,对测试的阴离子微生物表面活性剂的线性定量范围为10500μM.
- 在 *P. aeruginosa* 和 *B. thailandensis* 培养物中观察到时间依赖的生物表面活性剂积累.
- 缺乏生物表面活性剂生产的突变体在测试中表现出可以忽略不计的活性.
结论:
- 开发的基于纸张的测定方法提供了一种简单且易于使用的方法,用于量化水溶液中的各种生物表面活性剂.
- 该试验适用于在实验室环境中监测微生物生物表面活性剂的产生.
- 该方法为研究人员使用微生物表面活性剂提供了宝贵的工具.
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