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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jul 12, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

通过多色超分辨率膨胀显微镜增强突触蛋白质可视化.

Janna Eilts¹, Sebastian Reinhard¹, Nikolas Michetschläger¹

¹University of Würzburg, Department of Biotechnology and Biophysics, Biocenter, Würzburg, Germany.

|October 27, 2023

概括

研究人员开发了一种新的四色超高分辨率显微镜方法,用于突触蛋白质成像. 这种技术实现了20-30纳米的分辨率,为神经元网络组织提供了新的见解.

关键词:

10倍强大的扩展显微镜.扩张显微镜扩张显微镜多色成像技术的多色成像技术结构化照明显微镜结构化照明显微镜超分辨率显微镜的显微镜.突触蛋白质是一种突触蛋白质.

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Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

相关实验视频

Last Updated: Jul 12, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
显微镜的使用方法

背景情况:

了解生物分子复杂组织和动态对于细胞功能至关重要,特别是在神经元突触中.
超分辨率 (SR) 显微镜已经先进的突触成像,但在20-30纳米分辨率的多色成像仍然是一个挑战.

研究的目的:

开发一种方法,使20-30纳米空间分辨率的突触蛋白质能够进行多色光成像.
为了克服当前SR显微镜的局限性,进行详细的突触分子组织研究.

主要方法:

利用扩张后免疫标记八倍扩展的海马神经元.
联合扩展显微镜与Airyscan和结构化照明显微镜 (SIM).

主要成果:

在拥挤的细胞区内实现了对突触蛋白的高效标记,并实现了最小的链接错误.
展示了具有20-30nm横向分辨率的4色3D光成像.
确定了突触囊泡 (Synaptobrevin 2) 与突触前融合位点 (RIM1/2) 的同位点.

结论:

优化的扩展显微镜方法增强了突触蛋白的可视化和局部化.
提供了对神经元突触中的蛋白质空间组织的宝贵见解.
有助于更深入地了解突触功能和功能障碍.