相关实验视频
Updated: Jul 12, 2025

06:42
Extracting the Cochlea from a Human Temporal Bone: A Cadaveric Protocol
Published on: August 18, 2023
808
Mortui vivos docent:一个现代复兴的骨塞收获的现代复兴
Varun Sagi1, Nikitha Kosaraju1,2, Lindsay S Moore1
1Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, United States.
Frontiers in neuroscience
|October 27, 2023
概括
建立人类骨 (HTB) 处理管道加速了耳学研究. 优化的方法显著减少了脱时间,使内耳疾病的研究更快.
科学领域:
- 眼科病理学 眼科病理学
- 人体解剖学 解剖学 解剖学
- 历史学 历史学 历史学
背景情况:
- 人类骨 (HTB) 对于耳科研究至关重要,但它们的加工成本昂贵,技术要求高.
- 耳部病理实验室的减少阻碍了对耳部疾病的研究.
- 标准HTB处理是耗时的,限制了研究进展.
研究的目的:
- 概述一个精简的管道收集和处理HTBs.
- 减少HTB准备所需的时间和资源.
- 鼓励继续使用HTB进行耳科研究的研究.
主要方法:
- 设计了一种专门的刀,用于有效收集骨样本.
- 使用乙烯基胺三酸 (EDTA),微波处理和骨切割的优化脱,将时间从9个月缩短到3个月.
- 开发了一种快速钻孔技术,以访问眼囊,进一步将脱缩到几周.
- 在快速处理的HTB上对内耳蛋白进行验证的连续截面免疫染.
主要成果:
- 通过使用开发的管道成功收集和处理HTB.
- 实现了显著减少脱时间 (9个月到3个月,以及进一步到几周).
- 在快速脱的标本中证明了内耳蛋白质的成功免疫染色.
- 提供详细的方法与传播的视觉辅助工具.
结论:
- 建立的管道显著加快了用于耳科研究的HTB处理.
- 优化的方法使HTB研究更容易获得和更有效.
- 对HTB的持续研究对于了解和治疗耳部疾病至关重要.
相关概念视频
Sutures of the Skull
6.8K
The human skull is composed of several bones that come together to protect the brain and support the structures of the face. The junctions where these bones meet are called sutures.
Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...
Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...
6.8K
Cranial Bones: Lateral View
2.3K
The lateral view of the cranium is dominated by temporal, sphenoid, and ethmoid bones.
The temporal bone forms the lower lateral side of the skull. The temporal bone is subdivided into several regions. The flattened upper portion is the squamous portion of the temporal bone. Below this area and projecting anteriorly is the zygomatic process of the temporal bone, which forms the posterior portion of the zygomatic arch. Posteriorly is the mastoid portion of the temporal bone. Projecting...
The temporal bone forms the lower lateral side of the skull. The temporal bone is subdivided into several regions. The flattened upper portion is the squamous portion of the temporal bone. Below this area and projecting anteriorly is the zygomatic process of the temporal bone, which forms the posterior portion of the zygomatic arch. Posteriorly is the mastoid portion of the temporal bone. Projecting...
2.3K
Gross Anatomy of Bone
5.3K
The two main features of a long bone are the diaphysis and the epiphysis.
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in...
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in...
5.3K
The Auditory Ossicles
1.7K
The auditory ossicles of the middle ear transmit sounds from the air as vibrations to the fluid-filled cochlea. The auditory ossicles consist of two malleus (hammer) bones, two incus (anvil) bones, and two stapes (stirrups), one on each side. These bones develop during the fetal stage and are the ones to ossify first. They are fully mature at birth and do not grow afterward.
The aptly named stapes look very much like a stirrup. The three ossicles are unique to mammals, and each plays a role in...
The aptly named stapes look very much like a stirrup. The three ossicles are unique to mammals, and each plays a role in...
1.7K
Anatomy of the Ear
8.4K
Auditory sensation, commonly called hearing, involves the transformation of sonic waves into neural impulses facilitated by the structures of the auditory organ. The prominent, flesh-like structure on the side of the head, called the auricle, directs sound waves towards the auditory canal. The auricle is often mislabeled as the pinna, a term more aligned with mobile structures like a feline's external ear. The auditory canal penetrates the cranium via the external auditory meatus of the...
8.4K

