乳酸细菌衍生的外聚糖通过PC12细胞的NRF2-KEAP1通路缓解氧化反应
1Department of Biology, Faculty of Science, Gazi University, Teknikokullar, 06500 Ankara, Turkey.
Current issues in molecular biology
|October 27, 2023
概括
乳杆菌衍生的外聚糖 (L-EPSs) 显示出显著的抗氧化作用,通过调节NRF2-KEAP1通路,保护PC12细胞免受过氧化诱导的氧化应激.
科学领域:
- 神经化学 神经化学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 抗生素,包括来自乳杆菌物种 (L-EPSs) 的外聚糖 (EPSs),正在用于神经退行性疾病.
- 在PC12细胞中,L-EPSs对过氧化 (H2O2) 诱导的氧化应激的抗氧化能力仍未得到研究.
研究的目的:
- 为了研究L-EPSs的抗氧化特性.
- 为了确定潜在的分子点,并阐明L-EPSs的作用机制.
- 评估L-EPSs对神经细胞中H2O2诱导的氧化应激的影响.
主要方法:
- 来自Lactobacillus delbrueckii ssp. 的L-EPS的量化 布尔加里克斯B3和Lactiplantibacillus plantarum GD2使用光谱测量.
- 评估总醇/二硫化物平衡 (TDH),总氧化剂状态 (TOS) /总抗氧化剂状态 (TAS),抗氧化酶活性和细胞内活性氧物种 (ROS) 水平.
- 使用定量实时PCR (qRT-PCR) 和酶相关免疫吸收试验 (ELISA) 分析NRF2-KEAP1通路.
主要成果:
- L-EPS治疗显著增加了TAS,抗氧化酶活性和抗氧化基因 (GCLC,HO-1,NRF2,NQO1) 的表达.
- 使用L-EPS显著降低了TOS水平和KEAP1基因表达.
- 用L-EPSs治疗的PC12细胞显示出H2O2诱导的氧化应激标志物的显著缓解.
结论:
- L-EPSs具有显著的抗氧化特性,可以抵消PC12细胞中H2O2诱导的氧化应激.
- NRF2-KEAP1信号通路是L-EPS介导的抗氧化作用的关键目标.
- L-EPSs在神经化学中显示出治疗应用的潜力,特别是在管理与氧化压力相关的神经疾病方面.
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