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灵敏度均等化和动态范围扩展与多个光流体微泡共振器传感器.

Ye Wang1, Xuyang Zhao1, Liying Liu1

  • 1Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Fudan University, Shanghai 200433, China.

Biosensors
|October 27, 2023
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概括
此摘要是机器生成的。

一种新方法使光流体微气泡共振器 (OMBR) 的信号均等,从而实现直接比较并增强生物感知. 该技术实现了对HIV-1 p24抗原的广动态范围检测,显示了疾病诊断的潜力.

关键词:
艾滋病毒-1 p24 p24这就是WGM.生物感应生物感应这是光流体的光流体.

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科学领域:

  • 光流体学是一种光流体学.
  • 生物感应是一种生物感应.
  • 纳米光子学 纳米光子学

背景情况:

  • 光流体微泡共振器 (OMBR) 是一种敏感的生物传感器.
  • 在OMBR制造的变化可以导致不一致的传感信号.
  • 来自不同OMBR的信号的直接比较具有挑战性.

研究的目的:

  • 为OMBRs提出一种新的灵敏度均等方法.
  • 为了能够直接比较来自单个OMBR的传感信号.
  • 为了证明广泛的动态范围和可靠的生物传感用于HIV-1 p24抗原检测.

主要方法:

  • 开发了一种基于波长转移与散装折射率灵敏度 (BRIS) 的比率的灵敏度均等方法.
  • 实验验证该方法以确认信号均等.
  • 来自20个OMBR的集成信号实现了广泛的动态范围.

主要成果:

  • 均衡方法被证明是有效的,允许直接比较来自不同OMBR的信号.
  • 实现了HIV-1 p24抗原检测的广泛动态范围,从50 fg/mL到100 ng/mL (2.1 fM到4.2 nM).
  • 在传感信号中表现出良好的一致性和可重复性.

结论:

  • 这种新的均衡方法克服了OMBR中的信号变化.
  • 这种技术显著提高了实际生物传感应用的潜力.
  • 该方法对药物查和疾病诊断具有前途,以HIV-1 p24抗原检测为例.