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高压氧气和炎症对人类牙介质干细胞/祖细胞的影响

Johannes Tölle¹, Andreas Koch², Kristina Schlicht³

¹Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian-Albrechts-University, 24105 Kiel, Germany.

|October 27, 2023

概括

单次高压氧 (HBO) 治疗通过减少炎症和促进再生,有利于牙介质干细胞 (G-MSCs). 然而,在72小时内重复暴露于HBO是有害的,增加细胞应激和炎症.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
高压医学的高压医学.

背景情况:

牙介质干细胞 (G-MSCs) 对于牙周组织修复至关重要.
高压氧气疗法 (HBO) 正因其治疗潜力而受到探索.
需要阐明HBO对G-MSCs的影响,特别是在炎症条件下.

研究的目的:

研究单双HBO刺激对G-MSCs的影响.
分析G-MSCs的基因表达,通路激活,多能性和差异化潜力.
在炎症和非炎症环境中评估HBO对G-MSCs的影响.

主要方法:

从健康个体中分离和描述G-MSCs.
在实验性炎症条件下 (IL-1β,TNF-α,IFN-γ) 进行单 (24h) 或双 (72h) HBO刺激.
使用下一代测序,KEGG路径分析,多能性基因表达,Wnt/β-catenin路径激活,增殖,殖民地形成和分化试验进行分析.

主要成果:

单次HBO刺激显示出抗炎作用和诱导的分化 (TLL1,ID3,BHLHE40),增殖 (BMF,ID3,TXNIP,PDK4,ABL2),迁移 (ABL2) 和骨质分化.
在72小时后进行第二次HBO刺激,通过HMOX1,BHLHE40和ARL4C放大,加剧了由炎症引起的细胞应激和ROS积累.
G-MSCs表现出典型的间酶体干细胞/原生细胞特征.

结论:

短期,单次的HBO治疗对G-MSCs产生积极影响,提供抗炎和再生效益.
在72小时内重复HBO刺激对G-MSC特性产生不利影响.
研究结果表明,短期HBO在G-MSC介导的牙周再生中可能具有临床应用.

关键词:

这就是ROSOS ROS.高压氧气的高压氧气.这是一种炎症炎症炎症炎症.这是下一代测序.牙周炎是指牙周炎的一种疾病.复兴再生是一种再生方式.干细胞是干细胞的组成部分.

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...