循环外体circRNAs作为慢性冠状动脉综合征的诊断生物标志物
Xiaoyan Liu1,2,3, Meili Zheng1,2, Ruijuan Han4
1Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
Metabolites
|October 27, 2023
概括
血外体中的循环RNA (circRNAs) 在慢性冠状动脉综合征 (CCS) 中表现出不同的特征. Exo-hsa_circ_0075269和exo-hsa_circ_0000284是潜在的新生物标志物用于诊断CCS.
科学领域:
- 心血管疾病研究研究
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 循环RNA (circRNA) 参与了心血管疾病的发病.
- 外体circRNAs (exo-circRNAs) 作为慢性冠状动脉综合征 (CCS) 生物标志物的作用在很大程度上仍未被探索.
研究的目的:
- 为了研究CCS患者的血外体中circRNAs的差异表达.
- 为了识别潜在的外体circRNA生物标志物用于CCS诊断.
主要方法:
- 从15名CCS患者和15名非心脏性胸痛 (NCCP) 对照组的血外体RNA的高通量测序.
- 在独立队列中使用实时定量PCR验证选择的差异表达的exo-circRNA.
- 接收器操作特征 (ROC) 曲线分析以评估诊断潜力.
主要成果:
- 在CCS患者的血外体中,总共有276个circRNA被差异表达.
- 在CCS患者中,exo-hsa_circ_0075269和exo-hsa_circ_0000284的上调显著.
- 对于这两种生物标志物,ROC分析表明了诊断潜力 (AUC分别为0.761和0.623).
结论:
- 在CCS患者和对照人群之间,血外体circRNA表达特征显著不同.
- 血 exo-hsa_circ_0075269 和 exo-hsa_circ_0000284 显示为CCS的新型非侵入性生物标志物具有前途.
更多相关视频
相关概念视频
Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
Blood Studies for Cardiovascular System I: Cardiac Biomarkers
174
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
174
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
99
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
99
Acute Coronary Syndrome III: Diagnostic Studies
9
Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
9
Acute Coronary Syndrome I: Introduction
30
Acute Coronary Syndrome (ACS) encompasses a spectrum of heart conditions caused by sudden obstruction of coronary arteries, typically resulting from the rupture of an atherosclerotic plaque and subsequent thrombus (blood clot) formation. This obstruction can lead to partial or complete blockage of blood flow, causing varying degrees of myocardial ischemia or infarction.ACS includes the following clinical entities:Unstable Angina (UA)Non-ST-Elevation Myocardial Infarction (NSTEMI)ST-Elevation...
30
Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations
19
The pathophysiology of Acute Coronary Syndrome [ACD] involves several key processes:The main underlying cause of ACD is atherosclerosis, a chronic inflammatory disease characterized by the buildup of lipid-laden plaques within the coronary arteries.As the atherosclerotic plaque grows in the coronary artery, it may become unstable due to the formation of a lipid-rich core and a thin fibrous cap. Inflammatory cells within the plaque, such as macrophages, secrete enzymes that degrade the...
19


