随着时间的推移,在酒精中保存的和跳中,MALDI-TOF MS 个人资料的演变
Hanene Benyahia1,2, Philippe Parola1,2, Lionel Almeras1,2,3
1Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, 13005 Marseille, France.
Insects
|October 27, 2023
概括
矩阵辅助激光脱吸/离子-飞行时间 (MALDI-TOF) 质谱 (MS) 可以识别存储在酒精中的和跳长达四年. 优化干燥时间对于准确识别至关重要,特别是对于保存时间超过22个月的标本.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 矩阵辅助激光脱吸/离子化飞行时间 (MALDI-TOF) 质谱 (MS) 对于关节动物识别有价值.
- 在乙醇中储存外寄生虫可能会影响多发性硬化形状的稳定性和分类准确性.
研究的目的:
- 为了评估MALDI-TOF MS体 (Pediculus humanus corporis) 和猫 (Ctenocephalides felis) 在酒精中保存1至4年的稳定性.
- 确定储存时间和干燥时间对基于MS的识别准确性的影响.
主要方法:
- 测试了469个 (n=170) 和跳 (n=299) 的头胸部.
- 通过使用日志分数值 (LSVs) 对存储时间小于1年的标本的参考数据库进行评估的MS概况可重现性.
- 研究了延长干燥时间 (12-24小时) 对鉴定率的影响.
主要成果:
- 大约75%的和跳存储了12-48个月,取得了相关的识别.
- 存储超过22个月的标本的识别率显著下降.
- 延长的干燥时间提高了识别率,的识别率达到95%,跳的识别率达到80%.
结论:
- MALDI-TOF MS可以识别在酒精中保存长达四年的和跳.
- 足够的干燥时间对于准确的识别至关重要,特别是对于长期存储的标本.
- 优化的保存和干燥方案提高了MALDI-TOF MS在昆虫学研究中的实用性.
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