低温电产生高度多孔的纤维支架,从而提高了轨道网细胞透率
Devon J Crouch1, Carl M Sheridan1, Julia G Behnsen2
1Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool L7 8TX, UK.
Journal of functional biomaterials
|October 27, 2023
概括
低温电制造产生具有较大的孔径的生物模拟轨状状网状支架,改善了潜在的青光眼组织工程的细胞透. 这种方法更好地模仿自然组织结构.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 眼科医生 眼科 眼科
背景情况:
- 人类带状网 (HTM) 对于水性幽默的外流和预防青光眼非常重要.
- 功能障碍的HTM导致视力丧失,需要组织工程替代.
- 目前的组织工程方法需要复制HTM结构的仿生支架.
研究的目的:
- 开发一种生物模拟支架,用于使用电旋转的轨迹状网状组织工程.
- 为了比较传统的电与用于脚手架制造的冷电.
- 为了评估脚手架的结构性质和细胞透,用于潜在的青光眼治疗.
主要方法:
- 采用了传统的电和冷电技术.
- 脚手架的特点是毛孔大小,毛孔度和厚度.
- 在七天的时间内,评估了人体椎网状细胞系 (NTM5) 的附着和透.
主要成果:
- 由于冰晶的形成,冷电产生了由于冰晶形成而增加纤维间距的支架.
- 低温支架的结构特征 (毛孔大小,多孔性,厚度) 接近本地HTM.
- 细胞附着不受制造方法的影响,但冷支架促进了细胞深入透.
结论:
- 低温电是一个有前途的方法,用于创建生物模拟的状状网状支架.
- 这些冷支架显示出作为研究青光眼的先进3D体外模型的潜力.
- 这项技术对未来开发用于绿眼治疗的组织工程设备充满希望.
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