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五种Peltigera的完整线粒体基因组的表征和与相对物种进行比较分析

Gulmira Anwar¹, Reyim Mamut¹, Jiaqi Wang¹

¹College of Life Sciences and Technology, Xinjiang University, Urumchi 830017, China.

Journal of fungi (Basel, Switzerland)

|October 27, 2023

概括

测序了五种Peltigera物种的完整线粒体基因组,揭示了保存的编码区域和基因重排. 这些发现证实了线粒体蛋白质编码基因是可靠的研究Peltigera的基因.

科学领域:

* 菌群学 * 菌群学
* 基因组学是一个学科.
* 进化生物学进化生物学

背景情况:

* 皮尔蒂格拉物种是重要的形菌.
* 了解它们的进化关系对于分类学至关重要.
*线粒体基因组为真菌进化提供了宝贵的见解.

研究的目的:

*对五种Peltigera物种的完整线粒体基因组进行测序和分析.
* 将这些线粒基因组与相关物种进行比较.
* 评估线粒体蛋白质编码基因在Peltigera.内进行遗传学分析的实用性.

主要方法:

*对五种Peltigera物种进行DNA提取和测序.
*线粒体基因组组装和注释.
*对线粒基因组结构,基因含量和序列变异进行比较分析.
* 用14个编码蛋白质的基因进行了家族遗传学分析.

主要成果:

*获得了五个圆形线粒体基因组 (58,13269,325 bp).
* 分子基因组包含15个蛋白质编码基因 (PCG),2个rRNA,26-27个tRNA和一个ORF.
*观察到编码区域的高度保护,在基因间区域进行基因重排.
* *cox2*基因表现出最少的K2P遗传距离,这表明保存率很高.
*对11个Peltigera物种的遗传学分析产生了得到良好支持的拓.

结论:

* Peltigera 种类的线粒体基因组在编码区域中结构性保存.
* 基因在基因间区域的重新排列是Peltigera线粒体的特征.
*线粒体蛋白质编码基因是可靠的分子标记物,用于解决Peltigera的遗传关系.

关键词:

佩尔蒂杰拉 (Peltigera) 是一个进行比较分析.线粒体基因组的基因组

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