在韩国Candida auris隔离物中,病毒性特征和醇耐药性
Seung A Byun1, Yong Jun Kwon1, Ga Yeong Lee1
1Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea.
Journal of fungi (Basel, Switzerland)
|October 27, 2023
概括
这项研究发现,韩国Candida auris clade II分离物表现出较低的毒性和对可纳的耐药性,主要是由于Tac1Bp突变. 一级I分离物导致了韩国首次报告的医院病群.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 黄是一种新兴的多药耐药性真菌病原体.
- 了解它的毒性和抵抗机制对于感染控制至关重要.
- 韩国医院报告说,C. auris来自不同种群的分离物.
研究的目的:
- 分析韩国Candida auris分离的毒性特征和醇耐药机制.
- 为了调查C. auris.中弗卢可纳抗性的遗传基础.
- 为了比较不同类型的C. auris之间的毒性.
主要方法:
- 分析了来自韩国医院的104个Candida auris分离物 (1996-2022年).
- 确定对其他抗真菌药物的弗洛可纳耐药性和敏感性.
- 使用热耐受性,体外生长和Galleria mellonella模型进行病毒性评估.
- 在抵抗相关基因 (Erg11p, Tac1Ap, Tac1Bp) 中确定氨基酸替代.
主要成果:
- 分类II (96个分离物) 和分类I (8个分离物) 被确定.
- 在Clade II中具有较高的可纳耐药性 (68.8%),而在Clade I中为25.0%.
- 类II分离物体显示热耐受性降低,竞争性增长,以及较低的G. mellonella毒性.
- Tac1Bp突变 (特别是F214S) 在抗可纳的Clade II分离物中普遍存在.
- 克莱德I分离物与韩国 (2022) 的第一个医院C. auris集群有关.
- 所有的分离物都对安福特素B和类素敏感.
结论:
- 韩国C. auris的II类可能表现出较低的毒性,与其他类相比.
- 在Clade II分离物中,流可纳抗性的主要机制与Tac1Bp突变有关.
- 有效的治疗选择包括安福特里辛B和埃奇诺坎丁.
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