使用Trichoderma asperellum对抗Lasiodiplodia theobromae,导致Pomelo (最大) 的干末腐烂疾病
Nguyen Quoc Khuong1, Dinh Bich Nhien2, Le Thi My Thu1
1Faculty of Crop Science, College of Agriculture, Can Tho University, Can Tho 94115, Vietnam.
Journal of fungi (Basel, Switzerland)
|October 27, 2023
概括
研究人员确定了导致果茎末病的真菌病原体,并发现了用于生物控制的强效Trichoderma asperellum菌株. 这些发现提供了一种可持续的解决方案,以保护果生产免受这种破坏性疾病的影响.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 生物控制 生物控制
背景情况:
- 茎末腐烂病显著影响越南的葡萄果生产.
- 识别致病原体和有效的生物控制剂对于疾病管理至关重要.
研究的目的:
- 隔离和识别负责果茎末病 (PSERD) 的真菌病原体.
- 屏幕三色皮肤菌 spp. 对于在体外条件下对孤立病原体的对抗活性.
主要方法:
- 从越南本特雷省的有症状的葡萄果中分离出真菌病原体.
- 有五十个Trichoderma spp. 的种类. 来自当地土壤的菌株进行了双重测试,对PDA介质上的孤立病原体进行了双重测试.
- 通过ITS测序,分离物S-P06和S-P07被确定为Lasiodiplodia theobromae.
主要成果:
- 已经分离出11种致病性真菌导致PSERD.
- 拉西奥迪普洛迪亚神菌株S-P06和S-P07表现出快速菌生长.
- 在T-SP19和T-SP32的Trichoderma asperellum菌株中,对L. theobromae有很高的对抗效率 (84.7-86.2%).
结论:
- 拉西奥迪普洛迪亚 (Lasiodiplodia theobromae) 是导致PSERD的一个关键病原体.
- 特里科德玛阿斯佩雷勒姆菌株T-SP19和T-SP32是对PSERD病原体有效的生物控制剂.
- 这些选择的Trichoderma asperellum菌株显示出对果植物疾病的可持续管理的潜力.
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