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从Saccharomyces cerevisiae到乙醇:在代谢工程应用中释放进化工程的力量

Alican Topaloğlu^1,2, Ömer Esen^1,2, Burcu Turanlı-Yıldız²

¹Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Technical University, Istanbul 34469, Türkiye.

Journal of fungi (Basel, Switzerland)

|October 27, 2023

概括

面包酵母 (Saccharomyces cerevisiae) 是可持续生物乙醇生产的关键. 代谢和进化工程策略显著提高了乙醇产量,CRISPR-Cas9提供了未来的进展.

科学领域:

生物技术是生物技术.
微生物学微生物学
化学工程是化学工程的重要组成部分.

背景情况:

全球日益增长的能源需求和环境问题推动了对可再生生物燃料的研究.
乙醇是领先的生物燃料替代品,由于其低排放量和高度含量.
提高乙醇生产效率是研究的一个关键领域.

研究的目的:

审查代谢和进化工程策略,以提高Saccharomyces cerevisiae中的生物乙醇生产.
突出代谢和进化工程之间的协同关系.
讨论未来的方向,包括基因组进化技术的进步.

主要方法:

代谢工程:直接操纵参与乙醇代谢的基因.
逆代谢工程:一种自下而上的方法来改善菌株.
进化工程 (自适应实验室进化 - ALE):随机突变和系统选择以提高乙醇生产.

主要成果:

代谢和进化工程是强大的,往往交织在一起,提高生物乙醇生产的策略.
经过工程改造的Saccharomyces cerevisiae菌株显著提高了乙醇产量.
适应性实验室进化可以进一步优化通过代谢工程开发的菌株.

结论:

麦芽菌 (Saccharomyces cerevisiae) 是生物乙醇生产的重要微生物.
结合的代谢和进化工程方法提供了实质性的改进.
像CRISPR-Cas9这样的新兴技术对生物乙醇生产的未来进步充满希望.

关键词:

这种植物是Saccharomyces cerevisiae.适应性实验室进化 (ALE)生物乙醇生物乙醇的使用生物燃料是一种生物燃料.指导基因组进化是指导性基因组进化.乙醇生产乙醇生产乙醇的耐受性乙醇的耐受性进化工程是进化的工程.基因组编辑基因组编辑代谢工程是代谢工程.

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