在Pichia pastoris菌株作为强大的细胞工厂的代谢工程方面的进展
Jian Zha1, Dan Liu1, Juan Ren1
1School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Journal of fungi (Basel, Switzerland)
|October 27, 2023
概括
皮奇亚牧师是一种生产蛋白质和化学品的多功能酵母. 本综述详细介绍了其特性,遗传工具和生物合成中的应用,强调了细胞工厂发展的挑战和解决方案.
科学领域:
- 生物技术和合成生物学
- 微生物工程 微生物工程
- 工业微生物学 工业微生物学
背景情况:
- 皮奇亚牧师是工业和治疗性蛋白质生产的主要酵母.
- 它越来越多地被用作化学品和自然产品的细胞工厂.
- 了解其生理学和遗传工具对于优化其应用至关重要.
研究的目的:
- 为了审查Pichia pastoris的生理特性.
- 描述现有的遗传工具,包括CRISPR/Cas9和适应性实验室进化.
- 突出最近的进展,并讨论Pichia pastoris细胞工厂建设的挑战.
主要方法:
- 关于Pichia pastoris生理学和基因工程工具的文献综述.
- 对Pichia pastoris基于生物合成的近期成果的分析.
- 讨论目前Pichia pastoris细胞工厂的局限性和潜在战略.
主要成果:
- 皮奇亚牧草具有有利于生物生产的生理特征.
- 一套完整的基因工具套件有助于它的工程.
- 在其用于生产蛋白质,天然产品和其他化合物的使用方面取得了重大进展.
结论:
- 皮奇亚牧草是一种高度适应和强大的平台,用于各种生物生产应用.
- 对克服现有挑战的进一步研究将提高其作为细胞工厂的效率.
- 基因工具的持续开发将扩大其合成生物学能力.
相关概念视频
01:24Other Glycolytic Pathways
17
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
17
01:23Microbial Fermentation
35
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
35