时间与基质特定木材衰变的转录学在棕色红色真菌中Fibroporia radiculosa
Byoungnam Min1,2, Steven Ahrendt1,2, Anna Lipzen1
1US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Journal of fungi (Basel, Switzerland)
|October 27, 2023
概括
棕色腐烂真菌使用独特的酶来抑制木材腐烂. 基因表达分析揭示了基于木材形式和培养条件的独特酶激活,突出显示了真菌的适应性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 棕色腐烂真菌使用专门的机制降解木材,原因是缺乏素降解酶.
- 了解基因表达是阐明这些木材分解途径的关键.
研究的目的:
- 研究培养条件和木质基板对*Fibroporia radiculosa*的基因表达的影响.
- 为了确定不同条件下与木材降解相关的不同表达的基因.
主要方法:
- 培养 *Fibroporia radiculosa* 在磨木和实木晶圆上 树,松树和杉树.
- 进行比较转录组分析,以评估基因表达差异.
主要成果:
- 树木物种对真菌基因表达有很小的影响 (<3%的差异表达基因).
- 甘氨酸化酶和化酶的基因在木质晶片上升调节.
- 芬顿化学酶 (氨酸生物合成,氧化脱氧酶) 的基因在水下磨木培养物中被上调.
结论:
- 菌基因表达受到木质基质的物理形式的显著影响.
- 在木材腐烂的不同阶段,不同的酶途径被激活,表明真菌的代谢灵活性.
- 这项研究揭示了棕色腐烂真菌使用的各种木材腐烂策略的新见解.
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