全基因组识别Trachinotus ovatus抗菌和它们对两种病原体挑战的免疫反应

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Yu Liang^1,2, Jin-Min Pan¹, Ke-Cheng Zhu^1,3,4

¹Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.

|October 27, 2023

概括

金色庞巴诺水产养殖面临疾病威胁. 这项研究确定了黄金庞巴诺中的抗菌 (AMP),揭示了它们在免疫力中的作用以及开发新疗法对抗细菌和寄生虫感染的潜力.

科学领域:

基因组学和蛋白质组学
水产养殖和海洋生物学
免疫学和疾病抵抗力

背景情况:

黄金 (Trachinotus ovatus) 是一种有价值的水产养殖物种,但易受细菌和寄生虫疾病的影响.
了解鱼类的免疫系统,特别是抗微生物 (AMP),对于水产养殖中的疾病管理至关重要.
比较基因组学可以揭示鱼类免疫基因的进化模式.

研究的目的:

为了进行全基因组识别和表征抗微生物基因在金色的pompano.
分析鱼类中AMP的进化关系及其与物种进化的潜在联系.
研究AMPs在对细菌 (Streptococcus agalactiae) 和寄生虫 (Cryptocaryon irritans) 感染的反应中的表达模式.

主要方法:

对14种海洋和淡水鱼类的比较基因组学和遗传学分析.
使用APD3数据库识别和分类341个黄金的AMP.
在正常和感染组织中对AMP表达的转录组分析,加上丰富和PPI分析.

主要成果:

在金色庞巴诺中确定了341个AMP,TCP是最丰富的.
AMP分布在染色体之间有所不同,有重复事件的证据.
AMPs集中在疾病免疫路径中,它们的表达在感染期间显示组织特异性,可能涉及MAPK信号通路.

结论:

金色庞巴诺AMPs的全基因组识别为了解鱼类免疫提供了宝贵的资源.
增强型抗生素药物显示出有潜力作为向治疗药物来对抗水产养殖疾病,为传统抗生素提供了替代品.
对AMP的进一步研究可以加强抗病策略,改善水产养殖生产和可持续性.

关键词:

克里普托卡里昂刺激物链球杆菌 agalactiaeae 的情况.它们中的一个是Trachinotus ovatus.抗微生物 (AMP) 是一种抗微生物.基因表达的基因表达方式

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