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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Caspases01:24

Caspases

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Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jul 12, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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卡斯佩迪亚数据库:用于CRISPR-Cas酶类别2的功能分类系统.

Benjamin A Adler1,2, Marena I Trinidad1,3, Daniel Bellieny-Rabelo1,2

  • 1Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA.

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概括
此摘要是机器生成的。

CasPEDIA是一个新的数据库,分类了数百种Cas酶,包括Cas9,Cas12和Cas13家族. 该资源有助于研究人员理解和利用各种CRISPR-Cas系统来实现生物技术进步.

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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 基因组学就是基因组学.

背景情况:

  • 克里斯普尔-卡斯酶提供RNA引导的细菌免疫力,是基因组编辑技术的基础.
  • 第二类与CRISPR相关的酶 (Cas9,Cas12,Cas13) 在研究,医学和农业中具有广泛的应用.
  • 这些酶的巨大遗传和生物化学多样性给研究人员带来了挑战,他们的目标是利用它们的功能.

研究的目的:

  • 介绍CasPEDIA,一个对Cas效应蛋白质的精心策划的百科全书.
  • 为各种Cas酶提供标准化的分类和全面的信息资源.
  • 为了促进适合生物技术用途的Cas酶的选择和应用.

主要方法:

  • 开发CasPEDIA,一个基于Web的数据库 (http://caspedia.org).
  • 来自Cas9,Cas12,Cas13家族的数百种Cas酶的精选集成,以及相关的IscB和TnpB蛋白质.
  • 对于核酶活性,目标要求和指导RNA约束的标准化注释工作流.
  • 实行功能分类计划 (CasID) 与遗传学分类一起.

主要成果:

  • CasPEDIA为27个基因组的数百种Cas酶提供了酶分类.
  • 酶有核酶活性,目标特异性和指导RNA设计参数的注释.
  • CasID系统允许通过酶功能和序列相似性进行搜索,补充现有的遗传学命名法.

结论:

  • 卡斯佩迪亚 (CasPEDIA) 作为卡斯酶属性的综合数据门户.
  • 数据库将酶特征置于背景,帮助研究人员进行生物技术开发.
  • 卡斯佩迪亚使用户能够利用2类CRISPR-Cas酶的多样化的核酸向能力.