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细胞质中转移的ENDOG (内核酶G) 的生物功能
Wenjun Wang1,2, Jianshuang Li2, Qinghua Zhou1,2,3
1Office of Science and Education, The Sixth Affiliated Hospital of Jinan University (Dongguan Eastern Central Hospital), Jinan University, Dongguan, Guangdong, China.
Autophagy
|October 27, 2023
概括
在饥饿期间从线粒体中释放的Endonuclease ENDOG通过与关键蛋白质相互作用来触发自. 它还通过各种细胞转移调节亡,线粒和脂质合成.
科学领域:
- 线粒体生物学 线粒体生物学
- 细胞应激反应的细胞应激反应
- 分子信号通道的分子信号通道.
背景情况:
- 内核酶G (ENDOG) 主要以其在亡和线粒体DNA维护中的作用而闻名.
- 转移到细胞质的ENDOG的功能在很大程度上仍然没有特征.
- 之前的研究表明,ENDOG参与了饥饿时的自诱导.
研究的目的:
- 为了阐明细胞质转移ENDOG的生物学功能.
- 研究ENDOG调节自,脂质合成和其他细胞过程的分子机制.
- 了解ENDOG在各种压力条件下的不同角色.
主要方法:
- 酸化试验用于研究GSK3B的ENDOG修饰.
- 共同免疫沉用于分析蛋白质与蛋白质相互作用 (ENDOG-YWHAG/14-3-3γ,TSC2,PIK3C3/VPS34,RICTOR).
- 西方涂抹和细胞局部化研究以追踪ENDOG和相关蛋白质.
主要成果:
- 通过GSK3B对ENDOG的酸化增强了它与YWHAG/14-3-3γ的结合,释放了TSC2和PIK3C3/VPS34,抑制了MTORC1,并启动了自.
- 通过释放RICTOR和TSC2,ENDOG激活了MTORC2-AKT-ACLY通路,从而促进了乙-CoA的产生.
- 细胞质ENDOG与ER的HSPA5/BIP相互作用,激活ER应激反应并促进脂质合成.
结论:
- ENDOG表现出不同的细胞内定位 (线粒体,核,细胞质,ER) 取决于压力刺激.
- ENDOG通过不同的蛋白质相互作用来调节关键的细胞功能,包括亡,自,髓和脂质合成.
- ENDOG的多面性作用突显了它作为细胞应激反应的中央调节器的重要性.
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