发光剂的分子工程用于通过可激活聚合诱导的双色光通过多硫化的高完整性成像
Zhecha Zheng1, Jinzhu Gao1, Rongchen Wang1
1Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
ACS nano
|October 27, 2023
概括
研究人员开发了一种新型的化学选择性探针,以区分涉及硫化 (H2S) 的生物事件与它介导的生物事件. 这种成像工具使细胞内H2S的高完整性识别成为可能.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 区分与硫化 (H2S) 相关的生物事件与它介导的生物事件是至关重要的,但具有挑战性.
- 缺乏高完整性的成像工具阻碍了研究与H2S相关的生物过程.
研究的目的:
- 开发一种能够区分H2S和H2S的化学选择性探针.
- 创建一个生物相容和水溶性纳米探针,用于H2S的高完整性细胞内成像.
主要方法:
- 分子工程的发光剂,以创建一个chemoselective探针.
- 制造水溶和生物相容的纳米探针.
- 使用聚合诱导的双色光反应进行检测.
主要成果:
- 设计的纳米探针在588和750纳米处表现出罕见的聚合诱导的双色光,以响应H2S.
- 通过可激活的双色光技术,成功实现了细胞内H2S的高完整性识别.
- 探测器显示H2S对H2S的高选择性.
结论:
- 开发的H2S激活多色光探针为询问H2S介导的生物事件提供了有价值的工具.
- 这种方法为H2S在生物系统中的复杂作用提供了新的见解.
- 这些纳米探针由于其水溶性和生物相容性而适合于体内成像.
相关概念视频
Photoluminescence: Applications
410
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
410
Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K


