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基于生物信息学和在清细胞细胞癌中实验验验证的cuproptosis签名的构建

Xi Tian¹, Shuxuan Zhu¹, Wangrui Liu²

¹Department of Urology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China.

Journal of cancer research and clinical oncology

|October 27, 2023

概括

质亡是一种新的细胞死亡途径,影响清细胞细胞癌 (ccRCC) 的进展和抗瘤免疫力. 与cuproptosis相关的新特征可以作为ccRCC的治疗点.

科学领域:

在瘤学瘤学.
细胞死亡途径细胞死亡途径
癌症基因组学癌症基因组学

背景情况:

型亡是一种新发现的非亡细胞死亡途径.
它在清细胞细胞癌 (ccRCC) 中的作用尚不清楚.

研究的目的:

研究ccRCC中与cuproptosis相关的基因 (CRG) 的功能和预后意义.
探索cuproptosis和瘤微环境之间的关联.
评估cuproptosis作为ccRCC中的治疗点的潜力.

主要方法:

对881个ccRCC样本的基因表达,突变和临床数据的分析.
鉴定了16个与cuproptosis相关的基因 (CRG),并评估了它们的表达,变异和副本数量的变化.
利用单变体和多变体回归,非负矩阵因子化,基因组变异分析,CIBERSORT,ESTIMATE和ssGSEA来分析CRG,子组,生物功能和瘤微环境.
进行了体外实验 (CCK-8,Transwell,伤口愈合) 来研究DLAT的功能.

主要成果:

大多数CRG与ccRCC的整体存活率 (OS) 有积极的关联.
较高的cuproptosis得分,来自于一个新的签名,显着与更糟糕的OS相关 (p < 0.001).
杯性得分与免疫检查点分子表达 (PDCD1,CTLA4) 相相关,这表明对瘤透免疫细胞的影响.
在体外,DLAT的过度表达抑制了ccRCC细胞迁移和增殖.

结论:

在ccRCC中发现了一种与cuproptosis相关的新型特征.
质性特征可能通过复杂的调节网络调节抗瘤免疫力.
cuproptosis为开发ccRCC的新型治疗策略提供了一个潜在的途径.

关键词:

生物标志物生物标志物清细胞脏细胞癌.这种疾病叫做cuproptosis.预测预后预测预测瘤微环境是一个微环境.

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