相关实验视频

Updated: Jul 19, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

单细胞转录组分析揭示了与肝癌中巨细胞两极分化相关的关键基因

Zhenghua Ding¹, Zhongming Deng, Hengping Li

¹Department of General Surgery, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Hubei, China.

Hepatology communications

|October 27, 2023

概括

这项研究确定了六个关键基因 (CD53,TGFBI,S100A4,Pyruvate kinase M,LSP1,SPP1) 与肝癌中巨细胞极化有关,提供了潜在的治疗点. 这些基因还与巨细胞透和患者预后相关.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
遗传学遗传学是一个

背景情况:

肝癌微环境涉及复杂的免疫细胞相互作用.
巨细胞两极分化在肝癌的进展和免疫逃避中发挥着关键作用.

研究的目的:

为了确定与肝癌中巨细胞两极分化相关的关键基因.
探索这些基因在肝癌患者的预后价值.

主要方法:

利用了基因表达总体 (GEO) 和癌症基因组图谱 (TCGA) 数据集.
应用单细胞RNA测序分析 (Seurat 4.0) 用于细胞类型注释和极化评分 (SingleR).
执行了斯皮尔曼相关性和多变量考克斯回归来识别关键基因并构建预后模型.

主要成果:

确定了6个关键基因 (CD53,TGFBI,S100A4,Pyruvate kinase M,LSP1,SPP1) 与巨细胞极化显著相关.
这些基因与肝癌中巨细胞透水平呈正相关性.
一个基于这6个基因的预后模型有效地预测了患者的结果.

结论:

这些已识别的基因 (CD53,TGFBI,S100A4,Pyruvate kinase M,LSP1,SPP1) 是肝癌中巨细胞两极分化的关键调节者.
这些基因代表了肝癌治疗的潜在新型治疗点.
开发的预后模型有助于评估肝癌患者的预后.

更多相关视频

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Published on: January 7, 2019

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...