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猪流行性腹病毒的自然进化,从母体免疫的小猪中分离出来

Yufang Ge¹, Feiyang Jiang¹, Sibei Wang¹

¹College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.

Animals : an open access journal from MDPI

|October 27, 2023

概括

猪流行性腹病毒 (PEDV) 的演变给养猪业带来了挑战. 这项研究在中国发现了新的带有突变的PEDV菌株,有助于开发用于疾病控制的有效疫苗.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
动物卫生动物健康

背景情况:

猪流行性腹病毒 (PEDV) 在猪群中造成重大损失.
通过突变和重组的持续病毒进化使PEDV预防和控制复杂化.

研究的目的:

调查PEDV在中国的流行和进化特征.
从疫苗接种失败病例中分析PEDV菌株的遗传变异.

主要方法:

多重PCR,血素-欧染色和免疫组织化学被用于检测.
在Vero,PK-15和Marc-145细胞中进行病毒分离.
RDP4.0软件用于重组分析,然后进行测序和家族遗传分析.

主要成果:

检查材料中的PEDV阳性率为42.6%,影响肠道和淋巴结组织.
隔离的菌株在细胞培养中显示出较低的病毒标位.
遗传学分析显示,与疫苗菌株相比,PEDV SX6属于基因型GIIa,具有新的S区域突变和ORF3变异.

结论:

在中国鉴定出流行的GIIa PEDV菌株,具有显著的遗传变异.
这些发现强调了需要更新的疫苗,包括流行的野生型菌株.
这项研究支持加强中国的PEDV预防和控制战略.

关键词:

免疫学进化免疫学进化自然复合的自然复合物.猪流行病腹病毒病毒

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